Study On Chemical Constituents And Quality Evaluation Of Liparis Nervosa

Liparis nervosa is the whole herb of Liparis nervosa (Thunb.) Lindl. fromLiparis，Orchidaceae， which is a common folk traditional Chinese medicine. Themain function of Liparis nervosa is cooling blood，heat-clearing and detoxifying，it is commonly used to treat vomiting blood， coughing， sore swollen poison andbruise.The chemical components of Liparis nervosa are isolated and purified bycolumn chromatography， macroreticular absorbing resin， gas chromatography-mass spectrography(GC-MS).Spectrum and High Performance LiquidChromatography (HPLC) are used to identify compounds structure， studyfingerprint of Liparis nervosa and determine the content of Liparis nervosa flavones.The result of the research is that:1. The main chemical components of Liparis nervosa were clearifiedThere are20chemical compounds of Liparis nervosa were isolated and purifiedwith column chromatography， macroreticular absorbing resin and spectrum，according to the physicochemical properties and spectrum data， we can identify twonew compounds， one new natural product， and16compounds are isolated fromLiparis nervosa for the first time.2. The GC-MS analysis of Liparis nervosa were finishedThe different polar fractions of Liparis nervosa were analyzed by GC-MS，70peaks were detected and45compounds were identified， most of them were organicalcohol， organic acid， aldehydes ketones， phenols and ester compounds， somewere nitrogenous organic compounds， steroids and terpenoids were low.3. The fingerprint assay method of Liparis nervosa was establishedThe impact factor of HPLC map were investigated and fingerprint assay methodwere established: Welchrom-C18column(250mm×4.6mm，5μm)， mobile phaseacetonitrile(A)-0.2%triethylamine(pH adjusted to2.5with10%phosphoric acid)(B)，gradient elution，0~40min，87%~84%B；40~42min，84%~81%B；42~70min，81%~75%B， detect wave length was254nm， column temperature was30℃， flow rate1mL·min-1， sample volume was20μL. The fingerprint assay method of Liparis nervosa were established with Nervosine Ⅰas reference substance，the method had been proved stable and reliable.4. The HPLC determination method of two flavonoid constituents of Liparis nervosawas developedThe impact factor of HPLC map were investigated and determination method oftwo flavonoid constituents was developed: Welchrom-C18column(250mm×4.6mm，5μm)， mobile phase acetonitrile(A)-0.2%triethylamine(pH adjusted to2.5with10%phosphoric acid)(B)， gradient elution，0~40min，87%~84%B；40~42min，84%~81%B；42~70min，81%~75%B， detect wave length was254nm，column temperature was30℃， flow rate1mL·min-1， sample volume was20μL.The contents of two flavonoid constituents in ten batches Liparis nervosa was rangedfrom0.0735~0.2123%. The method had been proved stable and feasible.