Pharmacodynamic Studies Of Huzhang Jiangzhi Granules

Objective:To observe the effects of Huzhang Jiangzhi Granules(HJG) on acute hyperlipidemia in mice and hyperlipidemia in rats; to observe the effects of HJG on hemorheologic parameters in rats with blood stasis syndrome; to observe the effects of HJG on gastrointestinal motility in mice; to investigate the mechanism of regulating lipid of HJG.Methods:Acute hyperlipidemia in mice was induced by Triton WR-1339 to observe the effects of HJG on serum lipid levels. Hyperlipidemia in rats was induced by intaking of high fat emulsions to observe the effects of HJG on serum lipid levels, the levels of SOD, GSH-Px, MDA, LPL, HL in liver and hemorheologic parameters. Blood stasis syndrome in rats was induced by epinephrine injection and cold stimulation to observe the effects of HJG on hemorheologic parameters. The effects of HJG on gastric remnant rate and alvine pushing rate in normal mice and mice injected with atropine sulfate were observed.Results:1. For the acute hyperlipidemia in mice, preventive therapy of HJG with 12.60g·kg-1 and 25.20g·kg-1 doses respectively may markedly decrease the levels of TC, LDL-C and HDL-C in serum (P<0.05 or P<0.01), but had no effects on TG levels(P>0.05).2. For the hyperlipidemia in rats, HJG with 4.20g·kg-1, 8.40g·kg-1 and 16.80g·kg-1 doses was given for 30 days respectively. The results showed that HJG may markedly decrease the liver index, the levels of TC, LDL-C, HDL-C, AST, ALT in serum, but had no effects on the levels of TG, reatinine and urea nitrogen in serum(P>0.05). HJG may also decrease the levels of TC, TG in liver(P<0.05 or P<0.01). In addition, HJG may reduce the degree of hepatic steatosis.3. For the blood stasis syndrome in rats, preventive therapy of HJG with 4.20g·kg-1,8.40g·kg-1 and 16.80g·kg-1 doses respectively may markedly decrease hemorheology parameters including of whole blood viscosity, reduced viscosity, whole blood relative index at all shear rates, plasma viscosity, HCT, IER and IEA(P<0.05 or P<0.01). HJG also may markedly increase erythrocyte deformation index(IED) (P<0.01).4. For the normal mice and the mice injected with atropine sulfate, preventive therapy of HJG with 12.60g·kg-1 and 25.20g·kg-1 doses respectively may markedly deerease the-gastric remnant rate(P<0.05 or P<0.01) and increase the alvine pushing rate(P<0.05 or P<0.01).5. For the hyperlipidemia in rats, HJG with 4.20g·kg-1,8.40g·kg-1 and 16.80g·kg-1 doses was given for 30 days respectively. The results showed that HJG may markedly increase the levels of SOD, GSH-Px, MDA, LPL, HL in liver(P<0.05 or P<0.01). HJG may also decrease the levels of hemorheology parameters including of whole blood viscosity, reduced viscosity, whole blood relative index at all shear rates, plasma viscosity, hematocrit(HCT), erythrocyte rigidity index(IER) and erythrocyte aggregation index(IEA) (P< 0.05 or P<0.01). Furthermore, HJG may markedly increase erythrocyte deformation index(IED) (P<0.01).Conclusion:For the acute hyperlipidemia induced by Triton WR-1339 in mice, HJG plays a good role in lipid regulation. For the hyperlipidemia induced by intaking of high fat emulsions in rats, HJG can regular effectively the levels of serum lipid and liver lipid. HJG can effectively ameliorate hemorrheologic parameters of the blood stasis syndrome in rats; HJG can promote obviously the gastrointestinal movement in mice. The mechanism of regulating lipid of HJG might be related with the increasing the body’s antioxidant ability, increasing activity of LPL, HL and ameliorating hemorrheologic parameters.