| Objective: The carcinoma of larynx is one of the common malignant tumor in head and neck, around 93%-99% of which is laryngeal squamous cell carcinoma. Its incidence has a growth trend in recent years. Laryngeal carcinoma is divided into supraglottic carcinoma, glottic carcinoma, subglottic carcinoma and transglottic carcinoma based on its location and involvement, the most common type in which is supraglottic carcinoma and glottic carcinoma.At present, the pathogenesis of laryngeal carcinoma is still not very clear,and its occurrence, development and metastasis is a multi-stage process involving multiple genes and factors.recently, cancer research on the genetic level has become the attention of all scholars.Therefore, study on the genesis and development of of laryngeal carcinoma, especially the gene target with specific, is of great significance for the effective clinical prevention and treatment of laryngeal cancer.TPX2(targeting protein for Xenopus kinesin-like protein 2) and Aurora-A(serine/threonine-protein kinase 6), which are both the newly discovered oncogene, make remarkable contributions to centrosome maturation and spindle formation in cell mitosis and are closely related to the occurrence and development of tumors. However, the expression and clinical significance of the above factors in laryngeal carcinoma has not been reported. Our study plans to use the immunohistochemical method to detect the expressions of TPX2 and Aurora-A in laryngeal squamous cell carcinoma andpara-carcinoma normal tissues, analyse the relations and correlations between these expressions and clinicopathological factors,and explore the role of the two factors in the occurrence and development of laryngeal carcinoma.Methods: 42 laryngeal carcinoma samples and 42 para-carcinoma tissues are taken from the patients with laryngeal cancer who have accepted operation treatment in the Fourth Hospital of He Bei Medical University from February 2013 to September 2014. All tumor tissues are pathologically diagnosed as laryngeal squamous cell carcinoma, and without preoperative radiotherapy and chemotherapy.The expression of TPX2 and Aurora-A was detected by immunohistochemical SP technique in laryngeal carcinoma tissues(experiment group) and para-carcinoma tissues(control group). The results of experiment are analyzed by the SPSS19.0 statistical software. Test with P<0.05 has significant difference in statistics, and P<0.01 for the difference is very significant.Results:1 The expression of TPX2The positive expression of TPX2 was mainly located in the nucleus, and appeared brownish yellow. The high expression rate of TPX2 in laryngeal squamous cell carcinoma was significantly higher than that in para-carcinoma normal tissues. There was statistically significant between the two groups(76.2% vs 4.8%, P<0.05).The high expression rates of TPX2 in poorly differentiated, moderately differentiated, well differentiated carcinoma tissues were 91.7%、85.7% and 33.3%, respectively, and the expression of TPX2 was inversely correlated with the differentiation in laryngeal squamous cell carcinoma(P<0.05).The expression of TPX2 was related with the clinical stage.More advanced the clinical staging, more positive expression of TPX2. The positive rate of TPX2 in Ⅲ, Ⅳstage laryngeal carcinoma cases(95.0%) was significantly higher than that in I, Ⅱ stage group(59.1%, P<0.05).The high expression rate of TPX2 in the group with lymph node metastasis of cancer tissues was significantly higher than those without lymph node metastasis(94.4% vs 62.5%,P<0.05).However, The expression of TPX2 was not clearly correlated with age, sex, smoking history and tumor size of the patients(P>0.05).2 The expression of Aurora-AThe positive expression of Aurora-A was mainly located in the cytoplasm, and appeared brownish yellow. The high expression rate of Aurora-A in laryngeal squamous cell carcinoma was significantly higher than that in para-carcinoma normal tissues. There was statistically significant between the two groups(73.8% vs 7.1%, P<0.05).In poorly differentiate,moderately differentiated, well differentiated carcinoma group, the positive rate were 83.3%、81.0% and 44.4%, and the expression of TPX2 was inversely correlated with the differentiation in laryngeal squamous cell carcinoma, but no statistical difference(P>0.05).The expression of Aurora-A was correlation with the clinical stage. More advanced the clinical staging, more positive expression of Aurora-A. The positive rate of Aurora-A in Ⅲ, Ⅳstage laryngeal carcinoma cases was significantly higher than that in I, Ⅱ stage group(90.0% vs 59.1%, P<0.05).The high expression rate of Aurora-A in the cases with lymph node metastasis was significantly higher than those without lymph node metastasis(94.4% vs 58.3%,P<0.05).The expression of Aurora-A was not correlated with age, sex, smoking history and tumor size(P>0.05).3 Relations between TPX2 and Aurora-AThere was a positive correlation between the expressions of TPX2 and Aurora-A(r=0.430, P=0.004).Conclusion:1 The positive expression of TPX2 and Aurora-A in laryngeal squamous cell carcinoma was significantly higher than that in para-carcinoma normal tissues, which indicated that the two may be involved in the occurrence and progression of laryngeal squamous cell carcinoma.2 With the lower differentiation and more advanced clinical stage, the expression of TPX2 was higher, and positively correlated with lymph node metastasis, suggesting that TPX2 was closely related to the malignant proliferation of laryngeal carcinoma, and may be a marker of the biological characteristics of laryngeal carcinoma.3 The expression of Aurora-A was closely correlated with clinical stage and lymph node metastasis. The later clinical stage, the stronger expression of Aurora-A. And the high expression rate of Aurora-A in the cases with lymph node metastasis was significantly higher than those without lymph node metastasis, but not correlated with the differentiation. It was possibly that the overexpression of Aurora-A played an important role in the occurrence and progression of laryngeal squamous cell carcinoma.4 There was a positive correlation between the expressions of TPX2 and Aurora-A in laryngeal squamous cell carcinoma. It needs to be further studied and confirmed Whether combined detection TPX2 and Aurora-A is able to become a new targets for diagnosis and evaluation of laryngeal cancer in molecular level or provide the basis for a new mode of treatment of laryngeal cancer. |
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