| Gliomas is a common malignant tumor of the nervous system, with thehigh incidence of about46%. Due to the characteristics of glioma such asstrongly invasive, invasive tumor growth, rich in blood supply, the quickgrowth speed, high recurrence rate, and no clear boundary between normalbrain tissue, surgery and postoperative chemoradiation resistant, it is seriouslythreatening the health of patients. In order to improve the quality of life for thegliomas patients and expand the treatment methods, the research on gliomagradually become a hot spot,And correlative studies is gradually deepening.The formation of glioma suffered a series of complex process of change,whichglial cells were under the influence of multiple factors and varieties ofmechanisms. Life has been able to continue the final analysis, is constantlysplit due to the mother cell and constantly generation of the daughter cell. Weusually defined a cell cycle as a process which starts from the sub-cellgeneration to the next to form a sub-cell. Due to somatic cell proliferationmust suffer the process of mitosis, the cell cycle stability is particularlyimportant. Disorder of cell cycle, abnormality of chromosome replication,formation of polyploidy and aneuploid, all form into the glioma process. As aresult, Controling the change of tumor cell cycle are particularly important.During mitosis, chromosomes closely split need to rely on the spindle whichwork timely and effectively. And the spindle skeleton is made up of a varietyof biological functions of proteins (tubes). Microtubule formation is regulatedby a variety of related proteins, such as Xklp2target protein (targeting proteinfor Xenopus kinesin-like protein2, TPX2) and Aurora-A protein(serine/threonine-protein kinase6, Aurora-A), which are both the newlydiscovered kinds of microtubule associated protein, can stimulate and regulatethe maturity of the spindle, also the replication, maturity and divison of the cytocentrum. Now researchers found that the occurrence of breast cancer,ovarian cancer and esophageal cancer has great relationship with TPX2andAurora-A. Therefore, microtubule assembly and regulation play an importantrole in cell division and tumor formation.Objective: This study was to investigate the expression of PCNA(Proliferating Cell Nuclear Antigen PCNA), TPX2and Aurora-A in differentgrade gliomas. And to further disscuss the mechanism of TPX2and Aurora-Ain the occurrence and development of glioma, PCNA as a positive control.Method: In this study, using immunohistochemical method, we detectedthe expression of PCNA, TPX2and Aurora-A in50cases of human glioma ofdifferent grade and10cases of normal brain tissue (cortex decompression andfistula resection of brain tissue). According to WHO (2007revision) neuralepithelium tumor pathological diagnosis classification standards, randomlyselected lowgrade glioma for25cases, high grade ones for25and normalones for10. All preoperative specimens didn’t receive radiation andchemotherapy. A half quantitative analysis method was used to analysis theresults of the three, the positive staining results were graded. In the high fieldof vision (400x) five regions were randomly selected, each region counting100cells, nucleus and cytoplasm in yellow or tan color were defined as thepositive ones. Color evaluation is as follows:0represents without staining;1points represent weak staining;2points on behalf of the light shading; Threepoints on behalf of heavy shading. Cell evaluation are as follows:0representsno tumor cells;1points on behalf of the tumor cells was0%～10%;2pointson behalf of the tumor cells was11%～50%;3points on behalf of the tumorcells was more than50%. count the two evaluation scores together, Pointsbetween0-1write "-"; Points between2-3write "+"; Between4-5write "++";Between6-9write "+++". The cumulative value of the score determinedthe expression of the three cases, and then to analyze the relationship withmitosis, using SPSS17.0software for statistical process.Results: PCNA expression was not found in normal brain tissue, in thetissue of gliomas positive expression rate was68.0%(34/50), among them, there were12cases of grade II,22of grade III-IV (χ2=21.530, P <0.01),The expression of PCNA was statistically difference in normal control group,grade II and grade III-IV. Aurora-A expression was not found in normal braintissue. Positive expression rate was56.0%(28/50), among them, there were10cases in grade II,18cases in Grade III-IV (χ2=15.643, P <0.01). Aurora-aexpression between the three groups were statistically different. TPX2expression was not found in normal brain tissue, however, the positiveexpression of glioma tissues was62.0%(31/50), among them,11positivecases of the grade II,20of grade III-IV (χ2=19.315, P <0.01). TPX2expression between the three groups were statistically different. Aurora-A andTPX2expression in the glioma were positively correlated (r=0.521, P <0.01).Conclusion:1Aurora-A was highly expressed in glioma, especially in high-gradeglioma. And it wasn’t expressed in normal brain tissues. The gene is a newmarker and a new specific molechlar target for the evalution and treatment inthe glioma.2TPX2protein expression levels in the tumor tissue is significantlyhigher than normal brain tissue. And TPX2expressed more with the latertumor stage or worse histopathological grading. It indicates that the proteinhas close relationship with malignant proliferation of glioma.3PCNA is a common antigen of nuclear proliferation for tumormalignant evaluation,which expression level is associated with the grading ofglioma. Its expression level in glioma cells were higher than low grade ones.At present, PCNA protein as a marker of nuclear proliferation, which has beenapplied widly in tumor proliferation analysis. This study also confirmed thatthe PCNA is an important marker protein for brain glioma.4Aurora-A and TPX2are not expressed in normal brain tissue. But theyare highly expressed in gliomas expecially in high grade gliomas, whichmaybe contribute to glioma cell proliferation and invasive growth. The expression of Aurora-A and TPX2were related closely by correlation analysisin the gliomas tissues.5through follow-up confirmed, TPX2Aurora-A strong positive list ofatients from both negative or weakly positive expression in survivalsignificantly shortened, illustrate both the specificity of high expression maybe coordinated to promote disease and malignant progress of glioma, closelyrelated with the malignant degree and prognosis of glioma. |
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