| Objective:To investigate the influence of abnormal savdamunziq (ASMq) on the protein and RNA expression of transforming growth factor-betal (TGF-β1) in human hypertrophic scar fibroblasts (hHSFs) and clarify the mechanism of inhibiting the growth of hypertrophic scar at the molecular level. Methods:HHSFs were isolated from tissue specimens and cultured in vitro, and the cells at 3rd to 5th generation were selected for the further researches, which were cultured in DMEM culture media containing with different concentrations of ASMq (200,400,600 μg/mL). After culturing for 48 hours, the protein and mRNA expression of TGF-β1 in cells was examined by using Western blot and RT-PCR respectively. Results:Compared with the blank control group, the protein and mRNA expressions of TGF-β1 in cells cultured in the three doses of drug groups were decreased (P<0.05), and the effect of drug concentration at 400 μg/mLwas the best, meanwhile, the changes in protein and mRNA expression of TGF-β1 were consistency. Conclusion:ASMq could inhibit the expression of TGF-β1 in hHSFs cultured in vitro. This paper preliminarily discussed the action mechanism of inhibiting hypertrophic scar with ASMq, and provided a theoretical basis for the development and utilization of ASMq. |
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