Effect Of Uighur Medicine Abnormal Savda Munziq On The TGF-β₁ Induced In Hypertrophic Scar Fibroblasts

Background: hypertrophic scar is the result of over healing of the wound caused by excessive proliferation of fibroblasts, TGF-β₁ plays an important role in the related signaling pathway.ASMq has a strong inhibition effect on the proliferation in HFs, but its concrete mechanism is not clear. Objective: To investigate the effect of uighur medicine abnormal savda munziq on the TGF--β₁ induced in hypertrophic scar fibroblasts. Methods: Using tissue explant cultures of human hypertrophic scar fibroblasts, divided into six group: control group: add an equal volume of culture medium; and experimental group: treated with TGF-β₁ with or without different concentration of ASMq（0.2mg/ml,0.4mg/ml,0.6 mg/ml,1.0mg/ml）.CCK-8 assay was adopted to detect the proliferation of fibroblasts. western blot and RT-PCR were performed to detect the expression of CollagenⅠand Smad7. Results: ASMq reduced the TGF-β₁ induced in fibroblasts proliferation（P<0.05）; ASMq down-regulation the fibroblast expression of CollagenⅠmRNA and CollagenⅠinduced by TGF-β₁（P <0.05）, In addition, ASMq significantly increased the expression of Smad7 mRNA and Smad7 which was suppressed by TGF-β₁（P<0.05）. Conclusions: Our results demonstrate that ASMq blocks the TGF-β?Smad signalling pathway in hypertrophic scar fibroblast by up regulation of Smad7,, thereby reducing the proliferation of fibroblasts and synthesis of CollagenⅠ.