Effect Of Uighur Medicine Abnormal Savda Munzip On Cell Proliferation And Transforming Growth Factor Beta/Smad Signal Transduction Pathway In Fibroblasts Derived From Hypertrophic Scar

Objective: To evaluate the in vitro effect of abnormal savda munziq on the proliferation and TGF-β/Smad signal transduction pathway in hypertrophic scar fibroblasts.Methods:Data Hypertrophic scar fibroblasts were divided into six groups to receive different treatments as group A (blank control group), groups B-E (0.2,0.4,0.6,0.8,1.0 g/L abnormal savda munziq). Each group contained six specimens. After intervention, the MTT test, flow cytometry and immunocytochemistry methods were used to detect the alteration of cell proliferation and TGF-β/Smad signal transduction pathway.Results and Conclusion:The inhibition effect of abnormal savda munziq (0.2-0.8 g/L) on the fibroblasts enhanced in a concentration-dependent manner than the blank control group (P< 0.05). The immunocytochemistry results showed that the expression of Smad7 increased while expression of TGF-β1 decreased in hypertrophic scar fibroblasts with abnormal savda munziq. These findings suggest that the reduction on the proliferation caused by abnormal savda munziq could be attributed to its effects on TGF-β/Smad signal transduction pathway. Abnormal savda munziq could be used as an effective drug for treatment of hypertrophic scar.