Experimental Study Of Myocardial Protective Effect Of Danhong Injection On Experimental Acute Myocardial

Purpose: In this research, the author attempts to evaluate and analyse the anti myocardialischemia effect of Danhong injection and its mechanism for the treatment of ischemiccardiomyopathy, to further accumulate relevant basic experimental data.Method: This article adopts the method of heart coronary artery ligation in rats, induce,copy the experimental animal model of acute myocardial ischemia. Observation, evaluated theDan red injection of the animal model of myocardial ischemia ecg ST segment after thedeviation degree and serum myocardial enzyme activity (AST, LDH and CK-MB), serummyocardial troponin Ⅰ (cTn Ⅰ) content and the influence of the myocardial infarction area.Results: No.1, in the coronary artery ligation induced acute myocardial ischemia ECGST segment deviation degree of rats, for each group, Danhong injection of high dose(2.48ml/kg/d) and middle dose (1.24ml/kg/d) to offset the degree of ST segment of ECGanimal medicine group is significantly lower than that in ischemia group (all P<0.05);Danhong injection of low dose group (0.62ml/kg/d) animal ECG ST segment deviation degreeand ischemia model group has no significant difference (P>0.05), but there is a decreasingtrend. Control drug ligustrazine injection group (0.62ml/kg/d) offset degree animal ECG STsegment was also lower than the ischemia model group (P<0.05), but the Danhong injectionand ligustrazine injection had no significant difference between the two (P>0.05). Effects ofthree enzyme activity of serum myocardial rats by ligating the coronary artery of2on acutemyocardial ischemia in rats24h after coronary artery ligation, injection with salviamiltiorrhiza and safflower of high dose (2.48ml/kg/d), middle dose (1.24ml/kg/d) and lowdose (0.62ml/kg/d) group animal serum AST, LDH and CK-MB activity were significantlylower than those in model group (ischemia respectively P<0.05, P<0.01or P<0.001), andshowed a dose effect relationship. Control drug ligustrazine injection group (0.62ml/kg/d)animal serum AST, LDH and CK-MB activity were significantly lower than those in ischemiagroup (all P<0.05), but with the Danhong injection between each dosing groups had nosignificant difference (all P>0.05). After coronary artery occlusion effect of24h3onmyocardial ischemia induced by ligation of coronary artery of rat serum cardiac troponin I concentration, Danhong injection of high dose (2.48ml/kg/d), middle dose (1.24ml/kg/d)and low dose (0.62ml/kg/d) group animal serum cardiac troponins (cTn Ⅰ) was obviouslylower than the ischemia model group (respectively P<0.01, P<0.01and P<0.05), and on thebasis of the dosage of different showed a dose-response relationship. Control drug ligustrazineinjection group (0.62ml/kg/d) animal serum cardiac troponin I (cTn I) were also significantlylower than the ischemia model group (P<0.05), but with Danhong injection between eachdosing groups had no significant difference (all P>0.05). The area of myocardial infarction inrats by ligating the coronary artery of4on acute myocardial ischemia effects of Danhonginjection of high dose (2.48ml/kg/d) and medium dose (1.24ml/kg/d) to the area of myocardialinfarction animal medicine group were significantly smaller than the ischemia model group(P<0.01and P<0.05). Danhong injection in low dose group (0.62ml/kg/d) and the area ofmyocardial infarction and ischemia animal model group has no significant difference(P>0.05), but also has a certain reduction trend. Control drug ligustrazine injection group(0.62ml/kg/d) myocardial infarction area of animal is less than ischemia model group(P<0.05), but the Danhong injection and ligustrazine injection has no significant differencebetween the two (P>0.05).Conclusion: In the condition of myocardial ischemia, Danhong injection cansignificantly reduce the extent of ischemic ST segment deviation; effective inhibition ofmyocardial enzyme releases into the blood three, three reduction of serum myocardial enzymeactivity; content of the serum myocardial injury markers of cardiac troponin I decreasedsignificantly; and the coronary artery occlusion (ligated) induced by a significant reduction inthe range of myocardial infarction. So it has an obvious protective effect of ischemicmyocardial injury.