Chemical Constituents From The Roots Of Calotropis Procera

Calotropis procera belongs to the genus Calotropis of family Asclepiadaceae, which distributed in Yunnan, Sichuan, Guangxi and Guangdong provinces of China. It contains lots of kinds of compounds, such as cardenolides, flavonoids, terpenoids and sterides, which makes this plant has all kinds of pharmacological activities. As a traditional medicinal plant, C. procera has antitumor activity, vasodilatation effect, anti-inflammatory analgesic activity, antibacterial activity, antioxidant activity and et al, in which it is the focus of attention that cardenolides show the in vivo antitumor activity.To use the plant resource better and perfect the plant chemotaxonomy of the genus Calotropis, we have undertaken a systematic investigation on the chemical constituents of the roots of C. procera from Yunnan. Twenty-eight compounds were isolated from the plant and identified with the extensive spectroscopic technology including:1D NMR (’H-NMR,13C-NMR, DEPT),2D NMR (1H-1H COSY, HMQC, HMBC, ROESY), HRESI-MS, UV, IR etc. Sixteen of the compounds were unambiguously elucidated as:1-(1,3-dyhydroxyl-2-propoxy)-2-methoxy-4-(3-hydroxypropenyl)-benzene (1),3-hydroxymethyl-7-methoxyl-5-(3-hydroxypropenyl)-benzofuran (2), 1-methyl-3-(2-hydroxy-2-propyl)-cyclopentanecarboxylic acid (3), ω-hydroxy-propioguaiacone (4),1-O-methyl-guaiacylglycerol (5), Threo-85-7-methoxysyringylglycerol (6), C-Veratroylglycol (7),3,3’-Dimethoxy-4,8’-oxyneoligna-9,4’,7’,9’-tetraol-7-ene (8),9α-hydroxypinoresinol (11), (+)-syringaresinol (12),4,8-(3’-methoxy-4’-hydroxy)-diphenyl-2,6-dihydroxy-ditetrahydrofuran xyloge (13), Coroglaucigenin (14), Frugoside (18), Ficusol (26), p-hydroxyl-benzaldehyde (27), nicotinamide (28). Among them, compound 1-3 was new and compounds 4-8,11-12 and 26-28 were isolated from the plants of the genus Calotropis for the first time.