Isolation And Bioactivity Of Natural Products From Croton Caudatus Var. Tomentosus And Trametes Robiniophila

Croton caudatus Geisel. var. tomentosus Hook (Euphorbiaceae) and Trametes robiniophila Murr. (Polyporaceae) were chemically investigated and their anticancer activities have also been evaluated. A total of 38 secondary metabolites including 14 new ones were obtained after separation by classical column chromatography on silica gel, Sephadex LH-20, and C18 reverse-phased silica gel as well as preparative HPLC. Their structures were identified on the basis of NMR, MS, IR, and UV spectroscopy. Some of the diterpenoids from C. caudatus var. tomentosus were found to show weak cytotoxic activity.Fourteen new compounds, crotontomentosins A-M (1-13) and hexyl (Z)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (14), as well as nineteen know compounds, ent-15,16-epoxyhalim-5(10),13(16),14-trien-18-carboxylic acid (15), pomiferin D (16), pomiferin E (17),15-hydroxy-7-oxoabiet-8,11,13-triene (18), pomiferin F (19), margocin (20),16a,17-dihydroxy-ent-kaurane (21), annonebinide A (22),5-epi-eudesm-4(15)-ene-1β,6p-diol (23), dehydrololiolide (24), hexyl (E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (25),4-hydroxy-benzaldehyde (26), 4-hydroxy-3-methoxybenzaldehyde (27), ethyl 4-hydroxy-benzoate (28), 4-hydroxyacetophenone (29), β-sitosterol (30), stigmast-4-en-3,6-dione (31), β-sitostenone (32), and stigmastane-3,6-dione (33) were obtained from the twigs and leaves of C. caudatus var. tomentosus. Compounds 1-4 and 18 showed weak cytotoxic activity against the Hela, Hep G2, MDA-MB-231, and A549 cancer cell lines.Five know compounds, adenosine (34), ergost-7,22-dien-3β-o1 (35), ergosterol (36),3p-hydroxystigmast-5,22-dien-7-one (37), and daucosterol (38) were isolated from Trametes robiniophila Murr.. The structures were elucidated by NMR and MS. Compounds 34,35, and 37 were isolated from T. robiniophila for the first time.