Expression Of HIF-1α And VEGF MRNA In Fat Tissue Of Rats Under High Fat Diet And Intervention Effects Thereof On Aerobic Exercise

Purpose:To explore the effects of high-fat diet on the HIF-1tissue and VEGF and the impacts of aerobic exercise on obesity inflammation reaction and local hypoxia of adipose tissue using animal experiments, by analysis of changes with respect to Lee’s index, Plasma IL-6, TNF alpha, adipose tissue HIF-1α and VEGF mRNA expression level of rats before and after experiment.Methods:Select40eight-week old healthy and clean male SD rats, perform adaptive feeding for one week and randomly divide them into normal-diet no-exercise group (Group A), high-fat-diet no-exercise group (Group B), normal-diet exercise group (Group C) and high-fat-diet exercise group (Group D). Arrange them for aerobic exercise for six weeks, make them abrosia for twelve hours at the end of the sixth week and put them to death afterwards. Perform dissection, take out abdominal adipose tissues and insert such tissues in EP pipes for test. Sample blood at the tip of the heart to obtain separated plasma for testing and use the ELISA method to measure plasma IL-6and TNF alpha, the PCR method to measure adipose tissue’s HIF-lalpha mRNA and VEGF mRNA expression quantity and the western blot method to measure HIF-1α protein level in the adipose tissue. Finally, apply SPSS11.5software to process testing data obtained.Result:1. Weights of rats and their Lee’s index during experiment were on the rise and by the end of the sixth week, data level of all four groups were higher than that before the experiment (P<0.05, P<0.01).2.By the end of the sixth week, the double factor variance analysis of four group, weight,Lee’s index, fat cell diameter and visceral fat weight index display, high fat diet can increase the level of index.(P<0.05), and the movement an decrease the level of index(P<0.05). Diet combined with exercise without interaction (P>0.05). 3. By the end of the6week, the double factor variance analysis of four groups, IL-6, TNF-a of rats blood plasma, high fat diet can increase the level of the two indicators (P<0.05), and the movement can decrease the level of two indicators (P<0.05).Diet combined with exercise without interaction (P>0.05).4. Using fluorescent quantitative PCR to detect HIF-1α mRNA, each group signified existence of about468bp of specific bands, but the expression level of Group B and D were higher than that of Group A (P<0.05).Applying the western blot method to check HIF-lalpha, it was found that each experimental group had specific bands whose relative molecular mass were93kDa, but the HIF-1α protein expression level of Group B and D was distinctly higher than that of Group A.Taking use of fluorescent quantitative PCR to inspect VEGF mRNA, all groups were detected with about443bp of specific bands, but Group B, C and D had the expression level higher than that of Group A (P<0.05).Conclusions:1.6weeks of high fat diet, weight, Lee’s index increased, the increase of plasma IL-6and TNF-α levels, rats with obesity and inflammatory.2.6weeks of high fat diet can make the weight of rats visceral fat increased, and fat cell size increased significantly, HIF-1a protein of adipose tissue increased, adipose tissue with hypoxia; VEGF mRNA expression increased. Adipose tissue is an adaptive response to ischemia and hypoxia environment.3. Under high-fat-diet conditions, rats with aerobic exercise had relatively lower level of IL-6and TNF alpha, relatively smaller diameter of fat cells and relatively lower expression level of HIF-1α protein, HIF-la mRNA and VEGF mRNA in adipose tissues, which indicates that6weeks of aerobic exercise can reduce chronic inflammation reactions and reduce local anaerobic conditions in adipose tissues.