| Objective:With the improvement of the level of modern sport, the competitions become more and more fierce. In order to improve the performance of athletes, the loads of the athletes must increase during training. Therefore, the probability of fatigue appears higher and higher. New studies indicate that many of the protein hydrolyzes are active peptides that have a variety of physiological functions and anti-fatigue functions. Walnuts, as raw material in this experiment, peptides are extracted from them. The experiment explores the function of anti-fatigue of walnut peptide, and provides a theoretical basis for walnut peptide as athletes provide for the development of health food.Method:(1) Preparation of walnut peptideIn this study, walnut peptide was prepared by enzymolysis approach. Through experimental studies on different factors, I eventually chose neutral protease enzymes, accounts50%of the total, as the optimal enzyme and manufacture high quality walnut under optimal conditions.(2) The anti-fatigue functional experiments of walnut peptide on rats SD rats were randomly divided into the quiet control group, movement control group, low-dose group peptides, peptide dose group, and high dose peptide group. Gave them swimming training daily. After six weeks, let them have exhaustive swimming and recorded time.24hours after the exhaustive swimming, detected the blood lactate of the samples (LD), blood urea nitrogen (BUN), and hemoglobin (HB).Results:(1) Preparation of walnut peptideThrough experimental studies on different factors, peptides derived optimal preparation walnut hydrolysis conditions:enzyme concentration10000U/g, substrate concentration of10%, the hydrolysis reaction temperature50℃, pH7.5and the best optimum hydrolysis time180min.In this study, high-quality walnut peptide was prepared by the enzymatic method under optimal conditions. Reaction condition was mild. The quality was safe and reliable. The output was high and the color was light.(2) The anti-fatigue functional experiments of walnut peptide on ratsMedium and High doses of the peptide group’s swimming exhaustive time in motion is significantly higher than the control group (P<0.01); Rest for24hours, high medium and low doses of the peptide group blood lactic acid content in high movement were significantly lower than control group (P<0.01); high medium and low dose of peptide significantly below movement control group, the content of blood urea nitrogen (P<0.01); high medium and low doses of hemoglobin peptide group movement is significantly higher than the control group (P<0.01).Conclusion:Supplemental walnut peptide extended the exhaustion swimming time of rats, speed up the process of eliminating the lactic acid in muscle reduced the production of the serum urea nitrogen, increased the hemoglobin regeneration ability. It showed that walnut peptide can effectively promote the recovery of sports fatigue. |
