Bioinformatic Analysis And Identification Of Some Resistance-associated Genes Of ZF-HD Gene Family In Tomato

Tomato is famous for its excellent flavor,economy and research value.It is cultivated and promoted all over the world.At the same time,it is also a representative model plant in the process of crop genetic evolution research.It has now completed genome sequencing,which is for tomato genome level.The study provided convenience.Low temperature,drought,high salt and other adverse environmental conditions have a very important impact on the yield and quality of crops.Drought and soil salinization are serious problems in agricultural cultivation.In order to increase the yield and quality of agricultural products,and increase the efficiency of agricultural water resources and cultivated land,in addition to the application of new irrigation technologies,cultivation techniques,and soil improvement technologies,stress resistance Breeding and promotion of varieties are crucial.Therefore,it is particularly important to clarify the mechanism of plant stress response,explore the available stress-resistance resources,and explore ways of using stress-resistance gene resources in the selection and breeding of crop stress-resistance varieties.At present,studies on plant resistance have found that transcription factors activate or inhibit the expression of related genes through interaction with other related proteins or themselves,and thus play an important regulatory role in plant adaptation to adversity conditions..Members of the zinc-finger homeodomain(ZF-HD)family play a key role in the control of plant growth and development,which are involved in plant responses to stress.Although many functional studies of this gene family have been performed in different plants,the features of this family in tomato(Solanum lycopersicum)remain unknown.So,this study identified tomato ZF-HD genes and analyzed their gene structures,subfamily distribution,and expression characteristics.The results laid a foundation for tomato stress resistance breeding and the study of plant stress resistance mechanism.The experimental results are as follows:1 In this study,22 members of tomato ZF-HD family were identified by bioinformatics and divided into 7 subfamilies by system evolution analysis.The 22 members were located on 6 of 12 chromosomes in tomato by chromosome localization analysis.Finally,by analyzing the structural elements,it is proved that most of them are conservative;2 The results showed that there is high expression of SL-ZH1?SL-ZH6?SL-ZH14?SL-ZH15?SL-ZH17?SL-ZH19?SL-ZH20?SL-ZH22 in root,SL-ZH7?SL-ZH10?SL-ZH19?SL-ZH21 in stem,SL-ZH2?SL-ZH7?SL-ZH8?SL-ZH10?SL-ZH13?SL-ZH15?SL-ZH16 in the leaves,SL-ZH1?SL-ZH2?SL-ZH15?SL-ZH21?SL-ZH22 in flowers;3 In the screened 15 ZF-HD genes,different genes were involved in different adverse responses,SL-ZH?SL-ZH8?SL-ZH13 were related to cold resistance,SL-ZH8?SL-ZH10?SL-ZH13?SL-ZH15?SL-ZH16?SL-ZH17?SL-ZH21?SL-ZH22 were related to drought resistance,all 15 genes were related to salt tolerance;4 Two genes,SL-ZH13 and SL-ZH22,were used to verify the function by VIGS,the results showed that after silencing these two genes,respectively,the whole drought resistance and salt tolerance of plants decreased.