Theoretical And Experimental Study On The Effect Of Huatan Huoxue Fang In Experimental Hyperlipidemia Rats

Purpose:Objective to study the effect of Huatan Huoxue Fang on experimental hyperlipidemia in rats blood lipid, liver function and C-reactive protein, Huatan Huoxue Fang on regulating blood lipid effect is verified, and to explore its theoretical basis and mechanism of action.Method:1. Theoretical Study:the two major theoretical system of Chinese medicine and Western medicine, the causes and harm of hyperlipidemia are discussed in detail. Through literature review, analysis, expounds the understanding of the etiology and pathogenesis of hyperlipidemia, syndrome differentiation of TCM, review in recent years, of integrated traditional Chinese and Western medicine treatment principle and method of the latest progress. According to the research, the phlegm and stasis in hyperlipemia formation in the process of position, the Huatan Huoxue therapy hyperlipidemia prescription and syndrome relationship and drug action from the analysis of prescription compatibility significance.2. experimental study:the establishment of hyperlipidemia rat model, after 4 weeks of administration of drug administration, blood collection, blood test four, CRP reactive protein (C-), liver function, the calculation of atherosclerosis index (AI). And the liver tissue sections, HE staining. Changes of hepatic cell morphology in rats under microscope.Result:1. Theoretical study shows high fat disease etiology and pathogenesis of diet section, too, such as Yi less, emotional disorders, viscera function disorder, congenital deficiency, phlegm and blood stasis. The disease associated with the five internal organs, the final pathogenic factors of phlegm and blood stasis". According to the evolution of the disease, the experimental results show that the prevention and control of "phlegm" and "blood stasis" as the key to the disease, and suggested that the treatment of hyperlipidemia is a major means of treatment of hyperlipidemia.2. Huatan Huoxue Decoction can reduce high-fat hyperlipidemia rat serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) levels, reduce CRP index, atherosclerosis index (AI), hepatic and renal function than in the control group significant difference (P> 0.05). Light microscope observation of liver tissue sections found that phlegm and blood circulation can significantly reduce the fatty infiltration of liver in rats with hyperlipidemia.Conclusion:1. Theoretical studies show that the Huatan Huoxue Fang synergies, phase and Wu, in line with "disease and syndrome coherent" research ideas, has the effect of activating blood circulation and resolving phlegm, can be used for the treatment of hyperlipidemia.2. Through animal experiment research, Huatan Huoxue Decoction can significantly improve hyperlipidemia rats lipid metabolism abnormalities, decreased serum TG, TC, LDL-C content, reduce C-reactive protein (CRP) inflammation index, TC/HDL-C ratio, atherosclerosis index (AI) and can reduce the damage to the liver function of hyperlipidemia rats. It has the protective function to liver tissue, reduce the fat accumulation in the liver, reduce the fatty degeneration of hepatic cells, prevent the formation of fatty liver, which is of great significance in the prevention and treatment of hyperlipidemia.