The Change Of GSSG,GR In The Plasma Which Were Received High-voltage Electrical Burn And The Intervention Effect Of Ulinastatin

Objective: High voltage electric burn is a special injury induced by oxygen free radicals produced after burn(or)increase and decrease in antioxidant capacity caused by lipid peroxidation,protein oxidation,DNA damage and the damage of organs,tissues and cells are serious,serum glutathione content is one of the key factors to reduce oxidative stress injury.In the reduction reaction,GSH was used as electron donor to detoxify H 2 2 O,and to produce the final product oxidized glutathione(GSSG)(Gx).GR is a catalytic enzyme for GSSG reduction to GSH.Therefore,we designed this experiment aims to study electric burns in rats serum GSSG,GR changes,and the effects of ulinastatin(UTI)intervention,the mechanism of changes of GSSG and GR after burn,to evaluate the feasibility of UTI for high voltage electric burn.Methods:1 Animal groups: 240 of healthy adult SD rats(provided by the experimental animal center of Hebei Medical University Certificate.Animal permit number:SCXK(Ji)2013-1-003,No.832457),divided into four groups randomly,sham electrical burn group(referred to as: unelectrical group),high-voltage electric burn group(referred to as: electrical injury,high-voltage electric burn group)composite treatment group(UTI abbreviation: UTI Group),high-voltage electrical burn composite saline treatment group(referred to as: salt group,n = 60).According to the random number table each group was divided into six-phase group,15 min before electric shock,5min after electric shock,1h,2h,4h,8h after electric shock six-phase group.Each phase group was composed of 10 SD rats.2 Preparation: The rats were numbered,and weighed.Then shuck off therats' feather of left upper limbs,light legs and the chest.Experimental drugs prepared in accordance with the desired concentration.3 Modeling of high voltage electric burn: Connect the transformer and pressure regulator wire.Rats are anesthetized with 10% chloralhydrate by means of intraperitoneal injection,40 mg/kg administered.After the success of the anesthesia,put rats in special shock experiment-s on the stage,fixed limbs,then two 1cm×1cm electrode plate were fixed in rats' left upper limb(current entry),the right lower limb(currentexport)depilatory area.Switch on the power,adjust to boost pressure regulator of output voltage to 2 k V,connect the power of output,high-voltage electric gets through rat,shock time is 3s.The control group whichoffers only even wires and no electricity produce false electrical injurymodel.Rats with electric injury within 5 min,offer intraperitoneal injection of 5×104 U/kg UTI?250mg/kg MP in UTI group,and the salt group is injected of same amount of saline by means of intraperitoneal injection.4 Specimen collection and preservation:Operate the rat model which are replicated successful,open the chests,exposure heart,collect open-heart blood6 ml,Wherein is placed in a one-time use of vacuum vessels(red),and gently invert several times and stand for 30 min,put the serums in centrifuge,to3000r/min,centrifuge 10 min,take clear liquid in red tube to Eppendorf tube and preserve them in-70? conditions.5 Index test: Serum placed in the refrigerator was taken ELISA double sandwich antibody method to detect the GR and GSSG content.6 Analysis of Statistics:Using SPSS 21.0 statistical software to finish variance analysis of two factors factorial design,It has significant statistically meaning if P<0.05.Results:1 The changes of GSSG content in rats' plasmaGSSG contents in electrical injury groups are generally higher than those in unelectrical groups(P<0.05),and GSSG content over time after the injury showed significant differences(P<0.05),showing a rising trend,butthe change is more complex.GSSG contents in UTI treatment groups are generally lower than those in salt groups(P<0.05);and GSSG contents over time after the treatment showed significant differences(P <0.05),but only in the UTI group,the level of content in 5min after injury is higher than the value of this group before injury(P<0.05),2h,4h,8h after injury is no significant difference with the group of before injury(P<0.05).2 The changes of GR content in rats' plasmaGR contents in salt groups are generally higher than those in UTI groups(P<0.05),and GR content over time after the injury showed significant differences(P<0.05),and GR contents over time after the treatment showed significant differences(P<0.05).In the UTI groups after injury,from 1h to 8h,the level of content in each phase is higher than the value before injury(P<0.005).Conclusions:1 The expression of GSSG and GR in the early stage of high voltage electric burn were increased in different degrees.It was proved that the GSH consumed in the burned rats had a large amount of GSH to produce GSSG.At the same time,the reduction of GSSG by GR was needed to supplement the body.2 High voltage electric burn UTI group GSSG,GR and the salt content of the group decreased,suggesting that UTI treatment has antioxidant effect,can reduce GSH into GSSG,at the same time reduce the activity of GR,reduce the injury.