Influences Of High-voltage Electrical Burn On The Serum β-TG And PF-4 And Protection Of Ulinastatin In Rats

Objective:High voltage burns in addition to the immediate sexual electric injury,there is progressive damage of unknown cause.Studies have shown that microcirculation after electric burns of the progressive damage play a key role in the process of the occurrence and development of microcirculation after electrical injury and found that the main caused by microvascular injury and microflow obstacles.In the influence of microcirculation blood flow dynamics and rheology indexes,platelets play an important role rheological behavior.Is one of the major components of the blood platelet,it is the important factors influencing the microcirculation blood flow dynamics and rheology.High-voltage burns by stimulating the platelet activation,increase the platelet aggregation,adhesion,promote the release of reaction,so as to increase the microcirculation.But the molecular mechanism of platelet rheological behavior is not clear.This study by detecting high voltage early post burn serum of rats beta globulin platelet TG(beta)and platelet factor 4(PF-4)levels,and USES the ulinastatin(UTI)to intervene,to further explore high-voltage burns after platelet rheological behavior of related mechanism and method of prevention and cure.Methods:1 Group240 healthy adult male SD rats were randomly divided into false high voltage burn group(false),high voltage burn group(electric injury group),high voltage burns compound brine treatment group(saline group),high voltage burns composite UTI treatment group(UTI),each group of 60.Each group according to the observation time divided into injury before 15 min,5min after injury,the injury after 1 h,2 h,4 h,8 h six group,all the groups of10.2 ModelThe abdomen of the skin and disinfection.In rats by intraperitoneal injection of 10% chloral hydrate solution anesthesia(3 ml/kg),after the success of the anesthesia,fix the rats on special shock experiment platform,the two 1 cm * 1 cm of electrodes respectively fixed in rats left upper limb(current entrance),the right lower limb(current export).Connect the transformer and the voltage regulator electric wire,plug,regulating voltage regulator to 2 kv booster output voltage,turn on the booster output power,make the circulation of high voltage too big rats,shock 3 s,model making is successful.False wounded group only connect wires without power to false.By intraperitoneal injection of electrical injury within 5 min,UTI group 1%UTI(according to the 2 x 104 u/kg or 2 ml/kg dosing),intraperitoneal injection of saline group 2 ml/kg saline solution.3 Detection of indicatorsThoracotomy of model rats exposed to the heart,look straight into the heart blood 6 ml,in sterile plastic tube and let stand for 30 min,after waiting for serum precipitation on centrifuge,in 3000 r/min centrifuge for 10 min,take on in the clear liquid in Eppendorf tube-70 ℃ under the condition of preservation.Using the double antibody sandwich ELISA method,each group of rats was detected in six phases during beta serum TG,PF-4 levels.4 Statistical processing: using statistical software SPSS 21.0,line two factors factorial design analysis of variance,multiple comparisons using LSD method t test.With p < 0.05 for the test of significance level.Results:1 the rat serum beta TG content changesElectrical injury group serum beta TG content is higher than the average false injury group(F = 172.186,P < 0.01);Electrical injury group serum beta TG content after electrical injury time variable(F = 32.164,P < 0.01),the electrical injury after 1 h,2 h,4 h and 8 h value is higher than before the injury(P values are less than 0.01).Beta TG content in the serum of UTI group average lower than that of saline group(F = 21.295,P < 0.01);UTI betaserum TG content after electrical injury duration variable effects(F = 109.115,P < 0.01),1 h and 4 h after electrical injury when lower than that of saline group(P values are less than 0.01).2 In the rat serum PF-4 content changesElectrical injury group PF-4 content in serum is higher than the average false injury group(F = 29.108,P < 0.01);Contents of serum PF-4 electric injury after electrical injury time variable(F = 6.943,P < 0.01),2 h after electrical injury and 8 h value is higher than before the injury(P values are less than 0.01).UTI group PF-4 serum levels of average lower than that of saline group(F = 37.874,P < 0.01);UTI serum content of PF-4 electric injury after medication time variable(F = 15.026,P < 0.001),the electrical injury after 1 h,2 h,4 h,8 h below saline group(P values are less than 0.001).Conclusion: high-voltage burns can promote rat platelet release reaction,high voltage of rats caused by burn serum early beta TG and PF-4 content increases.UTI inhibiting rat platelet release reaction,make high-voltage burns in rats after plasma beta TG and PF-4 content is reduced,thereby improving the platelet rheological behavior.