| Objective: To objective the changes in mesenteric microcirculation of rabbits after high-voltage electric burn, to study the effect on microvascular and blood hydrodynamics by high-voltage electric and explore its mechanism, which may be offer theoretical foundation for the prevention and cure of organ injury after high-voltage electric burn.Methord: 1.Group: 30 adult healthy rabbits, 10 rabbits of which are used to preliminary experiment and others are randomly divided into two groups, high-voltage electric burn group (electric burn group) and fake high-voltage electric burn group (control group), 10 rabbits of each group, regardless of sex . The female rabbits have not been pregnant. 2.Preparation: These rabbits are weighted and ordered, then their hair of abdomen(10×6cm2) and outboard of left-up limbs and right-down limbs (5×5cm2)are cut after being anesthetized, after that these areas are epilated by 10% sodium sulphide solution. Rabbits are layed in 20℃~25℃ laboratories, dry naturally. 3.Duplication of high-voltage electric burn model: High-voltage electric system is made up of resistance box, step up transformer, voltage regulator, current transformer and electrostatic voltmeter. Anesthetized rabbits are put on the insulated platform and are joined into the 10,0000 volts high-voltage electric system for 5 seconds. Left-up limb is the entrance and right-down limb is the exit, electric current is 1.80±0.15A. The whole body muscles of rabbit convuled at the moment, and then skin, muscle, ligament in touch with the electrodes charcoaled after removing the electrodes. So high-voltage electric burn model is duplicated successfully. The progress of control group is the same as the electric burn group, but there isn't electric current in the body of control group rabbit. 4.Observation of mesentric microcirculation of rabbits : Abdomen epilated rabbit is put on experiment platform. A slang(6cm) is maked on the right musculus rectus abdominis, the abdominal is opened, mesentery is put out, and then an area which has microvascular and less fat is selected as the observing area. The mesentery is put out and spread on the irritate and flow box which is made of transparent lucite to be observed. Fully exposed mesentery microcirculation is observed with WX-9B poly-position microcirculation instrument and its results are taken down. 5.Time phase and index: The time phases of control group and electric burn group are divided into six phases, immediately, 0.5h, 1h, 2h, 4h, 6h.(They are recorded as T0, T1, T2, T3, T4, T5). Observing indexes are divided into microvascular morphology, microvascular flow state and microvascular peripheral state, amount to 13 indexes. 6.Analysis of resulta: Different kinds of results are dealed with by using SAS6.12 software.Results: 1.Mesentric microcirculation of every time phases of control group: There are no remarkable changes between different time phases of control group. The microvascular crooks naturely, the edge of it is smooth. The direction of microvascular blood flow can be distinguished clarity under microscope. The velocity of microvascular blood flow is normal. Erythrocyte aggregation are not observed in microvascular. There are not peripheral exudation and hemorrhagia around microvascular. Diastole and contraction of microvascular is normal. The cabilers of arteriole and veinule are 14.99±1.89μm and 28.59±2.86μm. 2.Oservation of different time phases after high-voltage electric burn: T0 time phase: Microvascular of rabbits mesentery is tortuous and cross slightly. Arteriole and veinule are contracted. The calibers of arteriole and veinule are 12.81±1.48μm and 25.91±2.83μm, which are different from that of control group(p＜0.05). The velocity of microvascular blood flow is simlar to the control group's. There is some peripheral exudation around microvascular. Hemorrhagia is not observed at this time phase. T1 time phase: At this time phase, microvascular is contracted stronghly and tortuous, a few stiff. Arteriole is contracted. The caliber of art...
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