| Objective: High voltage electric burn, which is a special kind of burns,can cause microcirculation of wound and distant tissue, resulting in hypoxiaand ischemia of tissue and organ, affecting the development and changes ofthe disease. The abnormality of blood cells rheological behavior is one of theimportant changes in microcirculatory hemodynamics disorders, and theabnormality of platelet rheological behavior play an important role in theoccurrence and development of microcirculation. High voltage electric burncauses vascular endothelium injury, and then platelet aggregation, with thecoming of release of inflammatory mediators, blood viscosity increasing, theformation of micro-thrombus, thus affecting the microcirculation blood flow.Thrombopoietin (TPO) and macrophage colony-stimulating factor (M-CSF)are important factors in regulating platelet production and major inflammatoryfactors in vivo. This experiment is designed to research the changes of TPO,M-CSF in the early of high-voltage burns rats, and through the intervention ofulinastatin (UTI) and methylprednisolone (MP), to further discuss themechanisms and interventions of the abnormality of platelet rheology behaviorinduced by high voltage electrical injury.Methods:1Group:240healthy adult male SD rats (provided by the ExperimentalAnimal Center of Hebei Medical University, certification No.1303144)were randomly divided into four groups according to the random number table,and60rats in each group. The groups were false high-voltage electricalburned group (control group), high-voltage electrical burned group(experiment group), high-voltage electrical burn-ed ulinastatin (UTI)treatment group (treatment group1) and methylprednisolone (MP) treatment group (treatment group2). There were six time phases for each group:ahead,immediate,1h,2h,4h,8h, and each time phase with10rats.2Preparation: Number and weigh the rats, and then shed the rats’feather of left upper limbs, right legs and the chest. The concentration ofexperimental drug required by experiment’s dubbed.3Duplicate the high-voltage electric burn model: Connect transformersand voltage regulators wire properly. Rats are anesthetized with1%sodiumpentobarbital by intraperitoneal injection, according to40mg/kg. After thesuccess of anesthesia, put rats on the special shock experiments stage, then fixlimbs with two1cm×1cm electrode pads fixed in the depilated area of rat leftarm (the current entrance) and the right lower extremity (current export)respectively. Switch on the power, adjust the output voltage of the booster to2kV, and then connect the booster so that high-voltage current gets through therats, shock and3s. The control group produce fake electrical injury model,which only connect the wires without electricity. Within5min after electricalburn, the rats offer intraperitoneal injection of5×104u/kg UTI in the treatmentgroup1,6ml/kg MP (250mg/kg) in the treatment group2, and the sameamount of saline in the control and experiment group.4Collection and detection: Operate the rat models that are replicatedsuccessfully, expose heart to collect open-heart blood6ml by using disposableVacutainer, invert gently several times, after standing for30min put the seruminto centrifuge at3000rpm/min for10min, and place the supernatant inEppendorf tubes,then store at-70℃.5Index detection: Apply ELISA double antibody method to detect thelevels of TPO, M-CSF in serum.6Analysis of Statistics: Use SPSS13.0statistical software to finishvariance analysis of two factors factorial design, multiple comparisons withLSD of t test method. It has significant statistically meaning if P<0.05.Results:1Changes of TPO Serum ContentTPO contents in experiment groups are generally higher than those in control groups (the main effect F=140.145,P<0.01), and they are affectedby time variable. There is a significant difference in phase groups (the maineffect F=7.747, P <0.01), showing a rising trend, but the change is morecomplex. each phase after electric injury, from5min to8h, is higher than thevalue of its group before injury (P <0.05).TPO contents in UTI treatment groups are generally lower than thosein electrical injury groups (the main effect F=47.827, P<0.01); and they areaffected by time variable. There is a significant difference in phase groups(the main effect F=27.264, P <0.01), showing a rising trend, but the changeis more complex. Each phase after electric injury, from5min to4h, is higherthan the value of its group before injury (P <0.05),8h after injury is nosignificant difference with the group of before injury (P>0.05).TPO contents in MP treatment group is generally lower than those in theelectrical injury group (the main effect F=276.764, P <0.01); and they areaffected by time variable. There is a significant difference in phase groups (themain effect F=5.632, P <0.01), after injury of8h in the MP treatment group issignificantly lower than the values before injury (P <0.05),5min、1h、2h、4hafter injury is no significant difference with the group of before injury(P>0.05).2Changes of M-CSF Serum ContentM-CSF contents in experiment groups are generally higher than thosein control groups (the main effect F=23.006, P<0.01), and they are affectedby time variable. There is a significant difference in phase groups (the maineffect F=3.391, P<0.01), showing a rising trend, but the change is morecomplex. Each phase is higher than the value of its group before injury expect8h (P<0.05),8h after injury is no significant difference with the group ofbefore injury (P>0.05).M-CSF contents in UTI treatment groups are generally lower thanthose in electrical injury groups (the main effect F=18.947, P<0.01); andthey are affected by time variable. There is a significant difference in phasegroups (the main effect F=4.494, P <0.01). but in the UTI treatment group, only the level of content in4h after injury is lower than the value of thisgroup before injury (P<0.05),5min,1h,2h,8h after injury is no significantdifference with the group of before injury (P>0.05).M-CSF contents in MP treatment group is generally lower than those inthe electrical injury group (the main effect F=7.182, P<0.01); and they areaffected by time variable. There is a significant difference in phase groups (themain effect F=3.571, P <0.05), but in the MP treatment group, only thelevel of content in8h after injury is lower than the value of this groupbefore injury (P<0.05),5min,1h,2h,4h after injury is no significantdifference with the group of before injury (P>0.05).Conclusion:1TPO contents of rats serum in early high-voltage electric burns haveincreased, showing an increasing trend in8h after injury. It proves thathigh-voltage electric burns induce platelet hemodynamic change,influencing blood flowing in the microcirculation.2M-CSF contents of rats serum in early high-voltage electric burnshave increased, showing an increasing trend in8h after injury. It proves thathigh-voltage electric burns promote inflammation in vivo.3TPO, M-CSF contents of rats serum have decreased in UTI and MPtreatment groups of high-voltage electric burns. It suggests that UTI andMP can suppress inflammation, improve blood clotting function, preventthrombosis to improve the microcirculation. |
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