Studies On Quality Evaluation Of Magnoliae Officinalis Cortex

Magnoliae Officinalis Cortex is the dry bark of Magnolia officinalis Rehd.et Wils.and Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils.,known as Houpo in China.It is a staple traditional Chinese medicine,which is documented by ancient herbal medicine and calendar version of "Chinese Pharmacopoeia".It has been used for thousands of years in China for its excellent traditional therapeutic effects on eliminating dampness and phlegm,promoting qi and removing distention.Modern pharmacological studies and clinical practice demonstrated that it possesses anti-inflammatory,antalgic,antioxidative,anticancer,antidepressant,and anti-diarrhea,etc properties.However,factors such as producing areas,harvesting times,and processing methods have a certain influence on the active ingredients of Magnoliae Officinalis Cortex,resulting in uneven quality of the medicinal material.So how to ensure the high and uniform quality of Magnoliae Officinalis Cortex in the production process of medicinal herbs has become a key issue.This project systematically analyzes the multi-index constituents of Magnoliae Officinalis Cortex,compared the differences in index constituents in Magnoliae Officinalis Cortex from different areas,different harvesting periods,different specifications,different medicinal parts with the same medicinal material,different processing methods and different "sweating" conditions;With the help of Metabolomics research ideas and methods,the topic studied on the chemical components differences by using UFLC-Triple TOF-MS/MS and GC-MS/MS technology.It compared that the compositions of "Chuan Pu" and“Wen Pu",different growth ages and harvested times,different specifications,"sweating" and "non-sweating",and screened and identified of the different chemical components;Fluorescence in situ hybridization(FISH)was used to measure the different stages of the“sweating”process of Magnoliae Officinalis Cortex,and the changes of microbial community structure at different stages during the process of "sweating" of Magnoliae Officinalis Cortex were compared.The endogenous strains of Magnoliae Officinalis Cortex were obtained by combining strain isolation and purification techniques,and UFLC-Triple TOF-MS/MS combined with multivariate statistical analysis techniques were used to the relativity between inoculation of Magnoliae Officinalis Cortex with different strains and“sweating”Magnoliae Officinalis Cortex and sterilized Magnoliae Officinalis Cortex.1.Literature Research Through systematically reviewing domestic and foreign related literatures,a literature review of the study was completed.2.Chemical Composition Analysis UFLC-Triple TOF-MS/MS and GC-MS/MS techniques were used to qualitatively analyze the chemical composition of Magnoliae Officinalis Cortex.The results showed that:A total of 24 non-volatile compounds were identified or tentative presumed in Magnolia Officinalis Cortex by analyzing the accurate mass of molecular and product ions provided by UFLC-Triple TOF-MS/MS,comparison with reference standards and the data of the literature;A total of 57 volatile components were identified by matching with the NIST05 database and relevant literature.3.Analysis of index components content in Magnoliae Officinalis Cortex Modern analysis techniques were used to compare and analyze the multi-index components of Magnoliae Officinalis Cortex from different origins and varieties,different specifications,different growth ages and harvesting times,different processing methods and“sweating”conditions,and gray correlation and principal component analysis were used to have a comprehensive evaluation.UFLC-QTRAP-MS/MS was established for simultaneous determination of lignans(magnolol and honokiol),alkaloids(betaine,magnoflorine),phenylpropanoid glycosides(syringin),flavonoid glycosides(rutin,quercitrin)and organic acids(chlorogenic acid)in Magnoliae Officinalis Cortex.GC-MS/MS technique was established to investigate the change of volatile components in Magnoliae Officinalis Cortex samples.A method was established for the determination of 24 inorganic elements in Magnoliae Officinalis Cortex by ICP-OES.The results showed that:The index components of Magnoliae Officinalis Cortex from different growth ages and harvested times showed dynamic changes;There were certain differences between the index components of different areas,different specifications,different processing methods and different“sweating”conditions.4.Studies on the plant metabolomics of Magnoliae Officinalis Cortex UFLC-Triple TOF-MS/MS combined with multivariate statistical analysis techniques were used to analyze the differences of non-volatile components in different areas and varieties,different specifications,different growth ages and harvested times,different processing methods and“sweating”conditions.GC-MS/MS combined with multivariate statistical analysis techniques were used to analyze the differences of volatile components in Magnoliae Officinalis Cortex from different origins and varieties,different specifications,different processing methods and“sweating”conditions.Component identification was based on the mass spectrometry data,combined with the software database and relevant literature.PC A and PLS-DA were used to find out the different significant chemical composition and their variation law in different metabolic groups.The results of PCA showed that the chemical composition of Magnoliae Officinalis Cortex in different metabolome groups were significantly different.Through further PLS-DA analysis.21 non-volatile components and 9 volatile components were screened out between "Chuan Pu" and "Wen Pu";10 common non-volatile components and 3 common volatile components were selected among different specifications;6 common non-volatile components were selected in different growth ages,and 7 common non-volatile components were selected in different harvest times,which show a dynamic trend;17 non-volatile components and 5 volatile components were screened out from "sweating" Magnoliae Officinalis Cortex and "non-sweating"Magnoliae Officinalis Cortex.5.To analyze the microbial community structure and its relevance to chemical conversion during the“Sweating" process of Magnoliae Officinalis Cortex In this experiment,FISH method was used to analyze and compare the species and quantity with the microorganisms of Magnoliae Officinalis Cortex in different periods of"sweating".Combining the strains with purification technology to obtain the endophytic bacteria in Magnoliae Officinalis Cortex.UFLC-Triple TOF-MS/MS combined with multivariate statistical analysis technology was used to analyze the correlation between inoculation of Magnoliae Officinalis Cortex with different strains and "sweating" Magnoliae Officinalis Cortex and sterilized Magnoliae Officinalis Cortex.It is still necessary to continue to explore the mechanism of quality formation of Magnoliae Officinalis Cortex and systematically explore the relevance of "sweating"and endogenous bacterial species.6.Analysis and comparison of chemical components in different medicinal parts of Magnolia officinalis UFLC-Triple TOF-MS/MS and GC-MS/MS techniques were used to analyze the chemical composition of Magnoliae Officinalis Flos.Moderm analysis techniques were used to compare and analyze the multi-index components of different medicinal parts of Magnolia officinalis,and principal component analysis was used to have a comprehensive evaluation.UFLC-Triple TOF-MS/MS and GC-MS/MS combined with multivariate statistical analysis techniques were used to analyze the chemical composition of different medicinal parts of Magnolia officinalis.The results show that:A total of 16 non-volatile components were identified or tentative presumed in Magnolia Officinalis Cortex by analyzing the accurate mass of molecular and product ions provided by UFLC-Triple TOF-MS/MS,comparison with reference standards and the data of the literature;A total of 46 volatile components were identified by matching with the NIST05 database and relevant literature;There are some differences in the multi-index components in Magnoliae Officinalis Cortex and Magnoliae Officinalis Flos;The results of PCA showed that the chemical composition of Magnoliae Officinalis Cortex samples and Magnoliae Officinalis Flos samples were significantly different.Through further PLS-DA analysis,18 non-volatile components and 15 volatile components were screened out between Magnoliae Officinalis Cortex and Magnoliae Officinalis Flos.