Effects Of Resistance Training And Endurance Training On AMPK/Sirt1 Signal Axis Of SD Rats Skeletal Muscle

The body can produce a large number of free radicals,such as reactive oxygen species,which have different effects on the body.The right content of reactive oxygen species can activate some signal factors in vivo,while the activation of AMPK and Sirt1 can promote glucose synthesis and fatty acid oxidation,so it maintains the energy metabolism of the body.The AMPK/Sirt1 signal axis affects the autophagy,apoptosis,cell proliferation and differentiation by regulating the downstream signal factors,and then decreases the damage of free radicals to the in vivo environment.Objective:The aim of research is to explore the effects of different exercise modes on the of effect reactive oxygen species,which regulates AMPK/Sirt1 signal axis by changing the activity of CaMKK beta and CaMK Ⅱ.The effects of aerobic exercise and resistance exercise on the phosphorylation of AMPK and Sirt1 activity in skeletal muscle of rats,which can regulate energy metabolism.It provides a scientific theoretical basis for us to develop a scientific exercise program to improve athletic ability and performance as well as to delay skeletal muscle attenuation.Methods:24 SD male rats(4 week)were randomly divided into control group(C,n=8),resistance training group(R,n=8)and aerobic training group(A,n=8).The "ladder climbing Pyramid" load,3 times a week,3 groups each time and different load resistance exercise and the slope is zero,the speed is 9m/min,30min/time,every five times a week treadmill exercise program.To obtain the corresponding experimental results by Reverse transcription polymerase chain reaction(RT-PCR)and Western blotting method and Elisa,and analyse the results of one way ANOVA by Excel and Graphpad5.0 software.Results:1.After the fourth week of A group rats weight was significantly higher than C group(P<0.05);Sixth weeks R group rats were significantly higher than A group(P<0.01);finally,the R group SD rats weight was significantly higher than A group(P<0.01).The quality of skeletal muscle of R group is significantly higher than C group(P<0.05).2.Compared with C group,the content of rat skeletal muscle ROS and RYR1 and NOS activity significantly increases in A and R group(P<0.01);compared with A group,the content of rat skeletal muscle ROS significantly increases in R group(P<0.01),the content of RYR1 significantly increases in R group(P<0.05),NOS activity has an increasing tendency in R group.Compared with C group,the content of rat quadriceps femoris muscle AMP increases significantly in A group and R group(P<0.01),the ratio of AMP/ATP increases significantly in A group(P<0.05),the ratio of R group significantly increases(P<0.01);compared with A group,the content of AMP significantly increases in R group(P<0.01),the ratio of AMP/ATP significantly increases in R group(P<0.05).Compared with C group,rat quadriceps femoris muscle Sirtl activity significantly increased in A group and R group(P<0.01);compared with A group,Sirtl activity significantly increases in R group(P<0.01).3.Compared with C group,the content of quadriceps femoris muscle CaMKK beta mRNA significantly increases in A group(P<0.01),the content of P-CaMK II thr286 protein significantly increases in R group(P<0.05);compared with A group,the content of CaMKK beta mRNA is significantly reduced in R group(P<0.05);the content of rats skeletal muscle CaMKK beta,P-CaMK II thr286 protein have an increasing tendency in C group and A group,R group.Compared with C group,the content of rat quadriceps femoris muscle AMPK alpha 1 mRNA significantly increases in A group(P<0.01);the content of AMPK alpha 2 mRNA overall have an increasing tendency in C group,A group and R group;compared with C group,the content of AMPK protein significantly increases in R group(P<0.01),and it is higher than that of A group;the ratio of P-AMPK/AMPK in C group,A group and R group has an increasing tendency.Compared with C group,the content of rat quadriceps femoris muscle Sirtl protein significantly increases in A group and R group(P<0.05),the content of Sirtl mRNA significantly increases in A group(P<0.05);the difference Sirtl protein and Sirtl mRNA between A group and R group has no significant.Conclusion:1.Resistance training can significantly increase the body weight and skeletal muscle mass.2.Resistance training and aerobic training can significantly increase the content of rat quadriceps femoris muscle ROS and RYR1 and NOS activity,and the expression content of CaMKK protein and CaMKIIthr286 phosphorylation increase,and the protein expression of AMPK and Sirtl and their activity increase,the effect of resistance training is greater than aerobic training.3.Resistance training and aerobic training can significantly increase the content of AMP and the ratio of AMP/ATP,they increase the oxidative stress effect,and then increase the content of reactive oxygen species,and increase the expression of CaMKK beta and CaMK Ⅱthr286,and finally regulate the activity of AMPK and Sirtl,and then actively regulate the energy metabolism.