Studies On Quality Of Different "Laba" Garlic And Functional And Thermodynamic Properties Of The Related Pigments

“Laba” garlic,a traditional product in Northern China,which is usually made with vinegar during winter.This paper used another way(dried fumigation)to prepare the “Laba” garlic,and to compare its sensory quality and biochemical properties with vinegar garlic,obtained another quick method.Then the purification of green pigment was carried out to prepare two kinds of pigment: yellow pigment and green pigment.The thermodynamic properties and functional properties of these pigments were studied.All the experimental results were e as follows:(1)The crispness properties,soluble solids,reducing sugar,total phenols,thiosulfinates content of the green garlic was decreased significantly compared to the fresh garlic;in scanning DPPH radical and ABTS radical,all products exhibited stronger antioxidant capacity.And the quality of the dried fumigation garlic exhibited stronger biological fumigation than vinegared garlic;(2)Thermal degradation kinetics of garlic yellow and green pigment was studied by isothermal heating at selected temperature(20?,40?,60?,80?)for different time(0h,1h,3h,5h,7h,9h).As a result,the yellow pigment and green pigment all exhibited stronger scanning DPPH and ABTS radical ability.And the scanning ability was decreased with the temperature and heating time.Decrease on temperature of the two kinds of pigments during heating time followed a first-order kinetic model.And temperature dependence of colour change was adequately modeled with the Arrhenius equation,to obtained the activation energy(Ea)were 19463.49(J/(mol*k)),29920.49(J/(mol*k)),respectivity.(3)The UV–vis absorption spectroscopy,fluorescence emission spectroscopy,viscosity,and ionic strength were used to research the interaction between garlic pigment(yellow pigment,green pigment)with Calf-DNA.Result showed with the pigments concerntration increased,the DNA UV–vis absorbation were increased,and company with red shift phenomenon;the fluorescence intensity of the pigment-DNA-NR was decreased;the viscosity of the pigment-DNA was increased;the fluorescence intensity of the pigment-DNA-Na Cl was decreased.Based above the reseach,It was conformed that the yellow pigment and green pigment interacts with ct-DNA all via minor embedded binding mode to protect the DNA from damage.