The Ingredients Difference And Indcible Expression Of Polysaccharide And Triterpenoids Among The Monokaryons And The Dikaryon Of Ganoderma Lucidum

Ganoderma lucidum has become used as a medicinal mushroom for thousands years in China.The nutritive value and pharmacological activities of Ganoderma lucidum have been exploited and developed through the effort of many researchers.And the pharmacological activities such as anti-cancer,anti-HIV,liver protection and lowered blood pressure are mostly related to the Ganoderma triterpenoids and polysaccharides.Recently,on the industry to obtain the Ganoderma polysaccharides and triterpenoids compounds is mostly from the mature fruiting bodies and mycelia of liquid fermentation.The liquid fermentation technology is easy to operate and the production cycle is short.Howerer,how to improve the content of the Ganoderma triterpenoids and polysaccharides through the liquid fermentation technology has become a very important research field.This study maintly contains the following parts.1.Comparing the growth rate of the monokaryons and dikaryon of Ganoderma lucidum.The result suggested that the dikaryon strain J-7/AL-2 growed faster than the monokaryons strain J-7 and AL-2.And comparing the biomass of the monokaryons and the dikaryon of Ganoderma lucidum.The result suggested the biomass of dikaryon was significantly higher than mononuclear strains in static cultures.But in shaking cultures the biomass of strain J-7 was match with the dikaryon strain J-7/AL-2.2.Comparing the intracellular polysaccharide and exopolysaccharides of the monokaryons and dikaryon of Ganoderma lucidum.The result showed that the intracellular polysaccharide and exopolysaccharides content of monokaryons strain J-7 was the highest in static cultures,But in shaking cultures,the intracellular polysaccharide and exopolysaccharides content of strain J-7 and J-7/AL-2 were almost the same but higher than strain AL-2.3.Comparing the triterpenoids content of the monokaryons and dikaryon of Ganoderma lucidum and dececting their fingerprint of Ganoderma lucidum triterpenoids by HPLC method.The results showed that the triterpenoids content of dikaryon strain J-7/AL-2 was both the most in static cultures and shaking cultures.And their fingerprints were also different.However,comparing the ganoderic acid E and ganoderic acid F of the monokaryons and dikaryon of Ganoderma lucidum in static cultures,we found that the content of ganoderic acid E and ganoderic acid F of monokaryon strain AL-2 was the most.Meantime,comparing the triterpeniods content by two cultivate method,the result showed that cultivated in static cultures was more conductive to the accumulation of triterpeniods.4.Comparing the different gene expression of six triterpeniods enzyme genes monokaryons and dikaryon of Ganoderma lucidum by qPCR method.We found that the expression of six triterpeniods enzyme genes in dikaryon strain J-7/AL-2 was much more than the monokaryons.5.We used CaCl2 and SA to induce the the Ganoderma triterpenoids and polysaccharides content and characterize the effect of CaCl2 and SA.The result suggested that the two inducer both could increase the content of the Ganoderma triterpenoids and polysaccharides as long as the concentration was suitable.And 10mM CaCl2 was more suitable to the to the accumulation of triterpeniods and polysaccharides,the add time had no effect.Also,150uM was more suitable to the accumulation of triterpeniods and polysaccharides,and the best induced time was the first and second time.6.Comparing the effect of the the Ganoderma triterpenoids and polysaccharides of the monokaryons and dikaryon induced by CaCl2 and SA.The result showed that the content of the Ganoderma triterpenoids and polysaccharides could be enhanced by two inducers in three strains but the inducible ability in three strains was different.7.Contrasting the induction of CaCl2?SA and the combination of CaCl2 and SA as the inducers to the triterpenoids content and enzyme genes expression.The result showed that three inducers could improve the triterpenoids content.Meantime,CaCl2 and the combination of CaCl2 and SA could enhanced six triterpenoids enmyze genes expression included HMGS?HMGR?MVD?FPS?SQS and LS.But SA could just enhance three triterpenoids enmyze genes expression included FPS?SQS and LS.The SQS gene expression was improved the most induced by three inducers.We guessed SQS may be the important gene in the GA biosynthesis...