Behavioral Responses And Application Of Yellow Sticky Traps Combined With Sex Pheromone On Diaphorina Citri (Hemiptera:Psyllidae)

The citrus psyllid(Diaphorina citri Kuwayama),belongs to Hemiptera Psyllidae is the main known vector pests that transmits huanglongbing(HLB)in nature,which is the most destructive citrus pathogen in the world.The control of citrus psyllid is an effective measure for the management of the citrus HLB.In order to reduce the use of chemical pesticides,we expect to explore the ecological control technology based on the combination of yellow sticky traps and insect sex pheromone.Firstly,the height,orientation and type of the yellow sticky traps were treated differently to explore whether they affected the citrus psylla trapping effects.Secondly,the effects of adult extracts of citrus psyllid on the selection behavior of males and females were analyzed by indoor behavioral bioassay and field trapping test.Furthermore,The constituents of adult extracts were identified by solvent extraction and GC-MS analysis.The effects of the compounds were also determined by indoor behavioral bioassay and field trapping test.The main results of the study were as follow:Observing the difference catches of citrus psyllid by setting the different height,orientation and type of the yellow sticky traps.Results showed that the height and the type of yellow sticky traps had a significant effect on the number of citrus psylla.The optimum height of yellow sticky traps is the upper edge flush with the top of host plants.The optimum adhesive substance of yellow sticky traps is tackifier.The effects of adult extracts of Citrus psyllid on their male and female behavior were measured by olfactory apparatus.Results showed that the male extract had no significant effect on the behavior of adults of Citrus psyllid;The female extract had a significant effect on the selection behavior of Citrus psyllid.Therefore,the female extract has the effect of attracting male citrus psyllid.We studied the effects of yellow sticky traps combined with adult extracts on the trapping of psyllids in the field.Results showed that there was no significant effect on the total catches of psyllids.However,there was a significant difference in the sex ratio of psyllids.When traps treated with female extracts,the catches of males were significantly higher than that of females.While treated with male extracts,there was no significant effect on females and males.The cuticular hydrocarbon of psyllid were collected by solvent extraction and their components were analyzed and identified by GC-MS.Results showed that 59 compounds were identified from the extracts of adults psyllid.The male extract contains 43 compounds,and the female extract contains 44 compounds.The contents of component A,component B and component C in the female extract were significantly higher than those in the male extract.The effects of different concentrations of component A in 100 μL n-hexane on the behavior of adults psyllid were measured by olfactory apparatus.Results showed that the concentration of 10 μg of component A on the male citrus louse has a seductive effect,while 0.1 μg and 1 μg doses of component A had no significant effect on the behavioral responses of females and males.We studied the effects of yellow sticky traps combined with different concentrations of component A on the trapping of psyllids in the field.Results showed that there was no significant effect on the total catches of citrus psyllid.However,there was a significant difference in the sex ratio of psyllids.When traps treated with female extracts 10 μg doses of component A,the catches of males were significantly higher than that of females.While treated with 0.1 μg and 1 μg doses of component A,there was no significant effect onfemales and males.