Melatonin On The Growth Cycle Of Hair Follicles In Inner Mongolia Cashmere Goats And On Wnt10b??-catenin Effect Of Gene Expression

This study was based on the skin samples of the adult ewes of the Inner Mongolia Albas White Cashmere Goat from January 2016 to December 2016,which were randomly divided into two groups,6 in each group.The melatonin was implanted at the rear and the control group was not implanted.Using the frozen section technique,the sections were analyzed by immunohistochemistry and HE staining to reveal the growth cycle of the skin hair follicles of Inner Mongolian cashmere goats and Wnt10 b,?-catenin The effect of catenin gene expression provides a theoretical basis for further exploring melatonin to promote the mechanism of villus growth in cashmere goats.The main findings are as follows:1.HE staining results show:In the same hair follicle growth cycle between the experimental group and the control group,the growth trends of the primary and secondary hair follicles were approximately the same,but there was a certain difference.Both groups entered the rest period in the 2nd to the middle of the month and the number of secondary hair follicles around the primary hair follicles Beginning to reduce and can obviously see the sebaceous glands;After the rest period,the primary hair follicles begin to become larger,the number of secondary hair follicles increases,and the hair follicles begin to enter the reconstruction phase.In the early growth period of May and June,the number of secondary hair follicles in the experimental group was significantly higher than that in the control group(P<0.05);the number of hair follicles in the experimental group and the control group was different before and after the prosperous period(September)in July.There was no significant difference(P>0.05).There was no significant difference in the number of secondary hair follicles between the experimental group and the control group before and after the decrepitude period(December)(P>0.05).2.Immunohistochemical results show:The Wnt10 b gene in the control group was mainly expressed in the upper and inner root sheaths of the hair follicle during the growth phase,while the Wnt10 b gene in the experimental group was also expressed in the hair matrix in addition to the inner and outer root sheaths.In the rest period,the control group Wnt10 b The gene was mainly expressed in the outer root sheath,but not in the inner root sheath and hair matrix.The Wnt10 b gene in the experimental group had a small amount of hair matrix except the root sheath.In the retrogression,the Wnt10 b gene in both the control group and the experimental group was expressed in the inner and outer roots.Sheath and hair matrix.The ?-catenin gene in the control group was mainly expressed in the outer root sheath of the hair follicle during the growth period,while the experimental group was also weakly expressed in the outer root sheath of the hair follicle and was also slightly expressed in the hair matrix.The ?-catenin gene was mainly expressed in the outer root sheath in the resting group,but not in the inner root sheath and hair matrix.The experimental group was expressed both in the outer root sheath and hair matrix.In the retrograde stage,the control group was expressed in the outer root of the hair follicle.Sheaths were expressed in the inner and outer root sheaths of the experimental group.From the above research results,it can be inferred that melatonin may lead to early development of secondary follicles in the early growth stage,induce secondary flock,and affect the expression intensity and expression position of Wnt10 b and ?-catenin genes at different stages of hair follicle growth.It is speculated that Wnt10 b and ?-catenin genes play a certain role in melatonin's alteration of the hair follicle cycle growth.