| With the improvement of people’s living standard,people pay more and more attention to natural extracts.Chinese prickly ash contains natural flavonoid polyphenol,this kind of material has certain health care function,but the study of Chinese prickly ash more focused on the essential oil and alkaloid,so for the flavonoid polyphenol content of prickly ash,composition,and active research is less.Taking the peel of pepper of fengxian da hong pao as the material,7 different solvents were selected and their polarity ranged from large to small.Methanol>ethanol>water>>ethyl acetate>chloroform>benzyl acetate,using ultrasonic oscillations auxiliary different solvent extract flavones in pricklyash peel polyphenols,using high performance liquid chromatography（HPLC）to extract the external standard method,choice of chlorogenic acid,rutin and quercetin,galangal,procyanidins b1,procyanidin b2 and catechins as a standard product,analysis of different solvents extraction,the differences in the composition and content of excavated extraction solvent of prickly ash polyphenol content of flavonoids,and tested the extracts antioxidant(DPPH free radical scavenging ability were measured and the reduction of Fe3+ability)and bacteriostatic(select filter diffusion method,The inhibition ability of candida albicans and staphylococcus aureus was determined.Major research results include:（1）Crude extract yield range between 8.44%and 27.54%,the yield of different extraction solvents from large to small is methanol respectively（27.54%）,acetic acid（25.75%）,alcohol（12.28%）,chloroform（10.07%）,ethyl acetate（9.57%）,water（9.36%）,benzene（8.44%）.（2）The total phenol content of crude extract of ethanol was the highest（82.29 mg/g）,while that of water was the lowest（56.46 mg/g）.The total flavone content of crude extract of methanol was the highest（110.69 mg/g）,while that of benzene was the lowest（22.42 mg/g）.In addition to water polarity,strong solvents such as methanol,ethanol extracted crude extract total flavonoids content is higher.（3）The crude extract of acetic acid detected the most ingredients（6 kinds）,including chlorogenic acid,rutin,quercetin,procyanidins b1,procyanidins b2 and catechins respectively.The crude extract of methanol was the least（3 kinds）,which were chlorogenic acid,quercetin and procyanidins b1.The contents of chlorogenic acid（0.10 mg/g）,quercetin（0.69 mg/g）and procyanidins b2（4.80 mg/g）of crude extract of acetic acid were higher than those of other crude extracts.The crude extract of benzene contained higher content of rutin（5.38 mg/g）than other crude extract.The content of procyanidins b1（3.02 mg/g）in crude extract of ethanol was higher than that of other crude extract.The content of catechin（0.70 mg/g）of crude extract of ethyl acetate was higher than that of other crude extract.Acetic acid is a good solvent choice for extraction of flavone polyphenols from pepper peels.（4）The crude extract of water had the strongest scavenging activity to DPPH free radical.Relatively polar solvents such as water and methanol extracted crude extract have strong REDOX activity to Fe3+.In general,the antioxidative activity of crude extracts with strong polarity extracted from strong solvents is strong.（5）The crude extract extracted with weak polarity solvent has strong antibacterial activity to candida albicans.For example,the crude extract extracted with chloroform has the strongest antibacterial activity to candida albicans.The crude extract extracted with intermediate polarity solvent has strong antibacterial activity to staphylococcus aureus,for example,the crude extract extracted with acetic acid has the strongest antibacterial activity to staphylococcus aureus. |
