Cultivation Of Coprinus Comatus With Crop Straw And Effects Of The Fungus Residue On The Growth Of Maize Seeding

Crop straw resources are rich and renewable resources,which makes up 60% of the biological yield of crops.Corn straw accounts for 27.39% of the total straw yield.It is great significant that utilizing of maize straw resources.Coprinuts comatus,a native grass-rotting fungus,has the characteristics of rich nutrition,cultivation easily and wide adaptability to raw materials.Using corn straw to cultivate Coprinuts comatushas a lot of advantages in realizing the effective utilization of resources,solving the pollution problem caused by corn straw,providing a new culture material for cultivating Coprinuts comatus and promoting the sustainable development of Coprinuts comatus indtry.The fertilizer made by fungus chaff of Coprinuts comatus can be used for maize cultivation,which has important practical significance and conforms to the development model of circular agriculture,apart from making full use of the fungus chaff,improving soil aggregate structure.Seven strains of Coprinuts comatus from different sources were selected in this paper.The lignocellulose decomposition ability of corn straw was compared by plate screening and enzyme activity detection using corn straw as carbon source.Then the optimum fomula of cultivating Coprinuts comatus was selected from the cultivation experiment of Coprinuts comatusfrom cultivation formula with different contents of corn straw.Finally,maize seedlings were potted in the soil which contained the fermented and composted funhus chaff.The suitable application amount of fungus bran fertilizer was screened out by analyzing various physiological and biochemical indexes,and soil physicochemical indexes during the growth of maize seedlings,In summary,the test results are as follows:1.Seven strains of Coprinus comatus were tested in this experiment(Coprinus comatus 01,Sanming,White Coprinuts comatus,cc100,White Coprinuts comatus 3,Te36,cc200).The degradation ability of lignocellulose in corn straw by plate screening and enzyme activity detection among 7 strains of Coprinus comatus.The results of plate screening showed that the strains with strong ability to degrade cellulose and lignin were Coprinus comatus 01,Sanming,CC100 and Te36.The results of enzyme activity detection including laccase,xylanase and carboxymethyl cellulaseshowed that Coprinus comatus 01 hasthe highest ability to produce three enzymes,and the enzymes activities were respectively 10.39 U/m L? 42.36 U/m L?3.30 U/m L.In this experiment,the optimum strain for degradation of maize straw was Coprinus comatus 01.2.The experiment of cultivating the Coprinus comatus 01 was carried out with 10 cultivating formulas of corn straw cultivation material.The cultivation formula which contains pure cotton seed hull was as control group(CK).The cultivation mode of bagging fermentation pot covering with soil and greenhouse cultivation.The growth of hypha of Coprinus comatus,the fruiting body yield,commercial character offruiting body,bio-conversion rate and lignocellulose degrading enzyme activity in mushroom bran before and after fruiting body growth were determined.The results showed that the Coprinus comatusmycelium growth rate were fastestin formula 7(60% corn straw,30% cottonseed hull,10% bran and 1.5% lime)and the shortest time of hyphae filling bag was 37 days,the highest yield was 1513.6 g.Besides,the commercial character of fruiting body was good,the bioconversion rate was 126.13%,and the lignocellulase activity had the biggest changes before and after fruiting body growth,which was significantly different from other treatments.Using corn straw to cultivate Coprinus comatus can reduce the cost of cultivating Coprinus comatus and make full use of agricultural resources.So formula 7 has good popularization value.3.The fungus chaff was fertilized and composted with adding dried chicken manure.The 11 fertilization levels were set,and the original soil culture was used as the control group.The treatment of applying fungus chaff fertilizer was compared with that of applying dried chicken manure.The results showed thatthe treatment with Coprinus comatus bran fertilizer could promote the growth of maize seedlings,which was more beneficial to the growth of maize seedlings than the treatment of dry chicken manure.T4 treatment had the most significant effect on the growth of maize seedlings in the experiment.The plant height of maize seedlings treated with T4 was 20.06% higher than CK.The fresh weight of maize seedlings treated with T4 increased by 39.8% compared with CK,and the chlorophyll content and photosynthetic rate of maize seedlings increased significantly.The further experimental results showed that fungus bran fertilizer could significantly reduce soil p H value and electrical conductivity(EC)effectively,improve saline-alkaline soil,increase soil organic matter,the content of available nutrient and total nutrient.According to the correlation analysis of plant height and dry weight of maize seedlings with soil physical and chemical properties.The results showed that the growth index of maize seedlings were significant correlated with soil EC value,organic matter,ammonia nitrogen,available phosphorus,available potassium,total nitrogen and total phosphorus content.The results showed that the contents of soil organic matter,ammonia nitrogen,available phosphorus and available potassium in T4 treatment were the highest,and the contents of total phosphorus and potassium in T4 treatment were the highest.Therefore,T4 treament was the best,and the fertilizer amount of Coprinus comatus fungus bran fertilizer per kilogram of soil was 20g.