| Objectives To detect the levels of interleukin-13(IL-13),interleukin-6(IL-6)and interleukin-17(IL-17)in children with mild to moderate bronchial asthma during acute attack,clinical remission and healthy children.To investigate the role of IL-13,IL-6 and IL-17 in the pathogenesis of bronchial asthma.Methods Selection of children(6-14 years old)who meet the diagnostic criteria for bronchial asthma at Tangshan People's Hospital from May 2016 to February 2017 as the study group.In accordance with the Guidelines for the Diagnosis and Control of Bronchial Asthma in Children(2016 Edition)Asthma group divided into acute attack group and clinical remission group.In the acute attack group,34 patients(20 men and 14 women)were 8±2.7 years old on average,while in the clinical remission group,26 patients(16 men and 10 women)were aged 6-14,with an average of 8.5±1.4 years.During the same period and in the same age group,30 children were examined in outpatient health checkups as control group.Testing for lung function in all groups of children.The expression levels of IL-13,IL-6 and IL-17 in serum of children were detected by enzyme linked immunosorbent assay(ELISA),FEV1 % and PEF % were recorded.The correlation analysis was carried out by Pearson rank correlation method,and the single factor variance analysis and T test were used to compare the quantitative data of each group.Results 1 Serum IL-13 level in children with acute attack of asthma 39.76±5.87.Serum IL-13 level in clinical remission group 26.79±4.29.Serum IL-13 in healthy control group was 24.75±5.05.Serum IL-13 levels in children with acute asthma were statistically significant compared with those in clinical remission and health control groups.(P<0.05).There was no significant difference between the level of serum IL-13 expression in children with asthma clinical remission group and the normal control group.(P>0.05).2 Serum IL-16 levels in children with acute attack of asthma 90.10±12.35.Serum IL-6 level in clinical remission group 49.76±12.81.Serum IL-6 level in healthy control group 46.89±10.79.Serum IL-6 levels in children with acute asthma were statistically significant compared with those in clinical remission and health control groups.(P<0.05).There was no significant difference between the level of serum IL-6 expression in children with asthma clinical remission group and the normal control group.(P>0.05).3 Serum IL-17 level in children with acute attack of asthma 89.82±10.33.Serum IL-6 level 68.17±8.25 in clinical remission group.Serum IL-17 level in healthy control group 65.46±8.915.The expression of serum IL 17 in acute attack of asthma was significantly different from that in clinical relief group and healthy control group(P<0.05).There was no significant difference between the level of serum IL-17 expression in children with asthma clinical remission group and the normal control group.(P>0.05).4 The level of IL-13 in children with acute attack was negatively correlated with lung function(FEV1(%)r =-0.532,P <0.05,PEF(%)r =-0.483,P <0.05).The level of IL-6 in children with acute attack is negatively correlated with lung function(FEV1(%)r =-0.495,P <0.05,PEF(%)r =-0.462,P <0.05).The level of IL-17 in children with acute attack is negatively correlated with lung function(FEV1(%)r =-0.500,P <0.05,PEF(%)r =-0.421,P <0.05).There was no significant correlation between IL-13 level and pulmonary function in children with clinical remission stage(FEV1(%)r = 0.254,P>0.05,PEF(%)r =0.281,P>0.05).There was no significant correlation between IL-6 level and pulmonary function in children with clinical remission stage(FEV1(%)r = 0.232,P>0.05,PEF(%)r =0.230,P>0.05).There was no significant correlation between IL-17 level and pulmonary function in children with clinical remission stage(FEV1(%)r = 0.201,P>0.05,PEF(%)r =0.143,P>0.05).Conclusions 1 The expression of serum IL-13,IL-6 and IL-17 increased significantly during the acute onset period of asthma in children with asthma.2 The expression of serum IL-13,IL-6 and IL-17 decreased significantly during the clinical remission period of asthma in children with asthma.3 The expression of serum IL-13,IL-6,IL-17 was inversely proportional to lung function in children with asthma during the acute onset period. |
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