Study On Relationship Between The Expression Of Oncogene Protein C-Met And Cell Proliferation In Cervical Squamous Carcinoma

Objective:To investigate the expression of oncogene protein c-Met and proliferating cell nuclear antigen(PCNA)in cervical intraepithelial neoplasia and cervical squamous carcinoma,and explore the relationship between the c-Met and the cell proliferation in the pathogenesis of cervical carcinoma to provide further theory evidence for its clinical therapy.Methods:The experimental subjects were divided into two groups:the experiment group were obtained from the cervical intraepithelial neoplasia tissue and cervical squamous carcinoma,and the normal control group were obtained from the cervical tissue of normal uterus,totally 96 cases,including 15 cases of normal cervical epithelium,18 cases of cervical intraepithelial neoplasiaⅠ,26 cases of cervical intraepithelial neoplasiaⅡ-Ⅲand 37 cases of cervical squamous cell carcinoma. Immunohistochemical technique(SP)were used to study the expression of c-Met protein and PCNA in each group.Results: 1.The positive granulas of c-Met protein were localized in the cytoplasm and/or cell membrane. Normal cervical epithelium was negative staining;CIN group was weak staining in basal layer and/or prickle cell layer;In cervical carcinoma group,the positive granulas were strongly staining around cancer nests,blood vessels and glands. The positive rates of C-Met protein of cervical carcinoma group,CINⅡ-Ⅲgroup and CINⅠgroup were respective 64.9%,46.2% and 11.1%.The difference was significant(P < 0.05).There was no observed variation between CINⅠgroup and normal control group. 2.The distribution of positive granulas of PCNA was clearly seen in the nuclear of cervical epithelium cell. Weakly staining was observed in the cytoplasm. The positive cells of PCNA of normal control group,CINⅠgroup,CINⅡ-Ⅲgroup and cervical carcinoma group were shown an upwardly tendency. The Proliferating Index(PI)of cervical carcinoma group(51.47±12.48) was predominant higher than other three group(P < 0.05). The difference of PI among CINⅡ-Ⅲgroup,CINⅠgroup and normal control group was also significant(P < 0.05).3.The expression of c-Met protein was related to cervical carcinoma clinicopathologic stage and histological differentiation. Strong staining was observed in low differentiation and advanced stage. The positive rates of c-Met protein between high differentiated cervical carcinoma and middle-low differentiation cervical carcinoma were shown significantly different(P < 0.05).The expression of c-Met protein in earlier stage carcinoma was far lower in advanced stage(P < 0.05).The upward tendency of PI was in accordant with the positive rates of c-Met protein.4.There was significant positive correlation between the expression of c-Met and PCNA in cervical squamous cell carcinoma.Conclusions: 1.The expression of oncogene c-Met was shown significantly different among normal control group,CINⅠgroup,CINⅡ-Ⅲgroup and cervical squamous carcinoma group. An upward tendency presented the relationship between the expression of c-Met and cervical squamous carcinoma.2.The expression of c-Met protein was related to cervical carcinoma clinicopathologic stage and histological differentiation which indicates that it can be a marker of prognosis for cervical squamous carcinoma.3.There was significant positive correlation between the expression of c-Met and PCNA in cervical squamous cell carcinoma. The oncogene c-Met may play an important role in the generation and progression of cervical carcinoma through cell proliferation.