| Objective:The subject passes the method that the alcohol infuses the stomach,building up the model of the poisoned big rat of the chronic alcohol,making use of the Morris circular water maze,the immunity set turn etc.the method and means,observing a the influence that stabs the big rat study memory ability to the chronic alcoholism and a HIF-1 expression,inquiries into the function mechanism of the treatment that the pinprick is poisoned to the chronic alcohol from the different level,develop the treatment function of the acupuncture,cures the work for the purpose of better instruction clinicMethods:Adopt the Morris circular water maze train,picking and get rid of the big rat that the swimming ability differ,sieving the big rat 100 of the normal male Wistar of a swimming ability,Random is divided into 25s of the blank matched control,the alcoholism a 75s.The alcoholism a big rat accordings to the chronic alcoholism big rat of allow the second prize making to build the mold method,adopting the density as 55%alcohol with 10 mls/kg/d(hang up to exchange the physiology brine to the 5 mls)infuse the stomach method carry on the alcohol to infuse the stomach to build the mold,the blank matched control adopts to infuse physiology brine of take the same physical volume(5 mls).Build to adopt the maze of Morris water to experiment to examine the big rat study memory ability before the mold and behind.Alcoholism rats die after making a successful model rats were divided into groups of scalp acupuncture group plexus,donepezil hydrochloride each group 20.The model set and the blank matched control give the day grab at once,infusing the stomach with physiology brine(5 mls)of the same physical volume in the meantime for day once.A cave cluster stabs a set of big rat give a cave cluster stabs the treatment,according to《the experiment animal acupuncture points diagram》take the big rat of 100 meetings and 100 meetings open 2 three acupuncture pointses of mm s beside,the needle and skin present 15°s inclined stab,stay the needle 30 mins,each 15 min 捻needle once,the frequency is a 100 rs/min,the 捻 turns 2 minutes each time.everyday is two,totally curing 4 weeks.Donepezil hydrochloride group of people and animals according to body surface area calculations translated to donepezil hydrochloride dissolved in 0.5%sodium carboxymethyl cellulose solution,0.05mg/kg orally administered to rats treated once a day,with the cluster of scalp acupuncture group treatment of rats in the same time period,four weeks of continuous treatment.Blank matched control,the model set:carry on to grab at to the big rat to bind the processing,the following day is once,the 30 mins of every time,total 4 weeks.After each big rat builds respectively before the mold and build the mold behind and cure 4 adopts the elusion incubation period that the maze of Morris water examines the big rat and wear the pedestal number of times after weeks to judge the study memory ability of the big rat.Make use of the change of the big rat sea horse area cell appearance that the HE dyeing method observes the chronic alcoholism model.A the sea horse CA1 area pathology variety and the expression of a HIF-1 that turns the big rat of the method observation.Statistical analysis of data using statistical software SPSS17.0,the behavior of rats in each group learning data and immunohistochemical determination of the value of HIF-1a of the proceeds are used to test the mean±standard deviation(χ±s)said,setting inside comparison adoption form couples the t examination,the an adopts the single factor relatively square bad analysis,square bad together after a 22 relatively adopt the t the examination,P<0.05 mean the difference has already statisticsed to learn the meaning.Results:1,the maze test of Morris water:(1)the space studies the ability:build before the mold,each time that big rat need when finding out the terrace namely evades the incubation period to carry on a set of’s compare,having no to statistics to learn the difference(P>0.05).Modeling six weeks,Cluster Scalp Acupuncture group,model group,donepezil hydrochloride escape latency than the control group prolonged compared with statistical significance(P<0.05)compared with the control group;Before modeling,behind barbed point cluster group,model group,the escape latency donepezil hydrochloride groups of rats were compared statistically significant(P<0.05).After 4 weeks of treatment,Cluster Scalp Acupuncture group and the donepezil group compared with model group,escape latency were shortened statistically significant(P<0.05);Cluster Scalp Acupuncture group and the donepezil group compared escape latency times were shortened no significant difference(P>0.05),no statistically significant.(2)spatial memory:In space exploration experiments with the model rats were compared Cluster Scalp Acupuncture group and the donepezil group were significantly increased wear station number,with statistical significance(P<0.05),Cluster Scalp Acupuncture group and donepezil group difference was not significant(P>0.05).2,HE staining:After the rats were stained blank obvious pathological changes,most of the model group were stained neurons obvious shrinkage,cell bodies of various shapes,including pulp and concentrated,dark stained nucleolus shrink or even disappear,neurons appeared shell surrounding area;cluster Scalp Acupuncture group and the donepezil group of nerve cells form a more complete,occasionally pyknotic cells,compared with the model group were the extent of damage lighter Cluster Scalp Acupuncture and donepezil group,which pyknotic cells than donepezil group.3,HIF-1a expression:Immunohistochemical blank group occasionally HIF-la-positive cells,model group,Cluster Scalp Acupuncture group,donepezil hydrochloride group were seen HIF-1a expression increased,with the control group and the difference was statistically significant(P<0.05);Plexus of scalp acupuncture group and donepezil hydrochloride low expression group(P<0.05)compared with the model group.Conclusions:1,through the Morris water maze test of scalp acupuncture group and cluster donepezil hydrochloride group can make chronic alcoholism rats escape latency and increase the number of units to wear,improves spatial learning and memory in rats with chronic alcoholism,and both considerable effect;2,by HE staining,pathological changes of chronic alcoholism from cytological rat hippocampal CA1 region,the degree of destruction of scalp acupuncture group and cluster donepezil group of nerve cells in the form of a lesser degree than the model group;3,suggesting Cluster Scalp Acupuncture may affect the expression of brain tissue to protect the role of HIF-1a and reduce damage brain tissue,thereby changed chronic alcoholism learning and memory in rats. |
PDF Full Text Request