Experimental Study Of Polycarbonate/gelatin Microspheres Promoting Proliferation And Adhesion Of Bone Marrow Mesenchymal Stem Cells

Objectives:In this study,a high–voltage electrostatic technique is introduced to prepare polycarbonate(PC)microspheres in order to design an expansion strategy for the bone marrow mesenchymal stem cells(BMMSCs).Therefore,it provides a new method to solve the problem of BMMSCs deficiency in clinical bone defect treatment.Methods:The effects of the solution concentration,temperature,nozzle specification and voltage on the sphericity,homogeneity,diameter and sedimentation velocities of the microspheres are investigated.BMSCs were respectively cultured on the surface of the optimal microspheres and the modified microspheres to observe cell growth and amplification.Results:By optimising the preparation parameters,PC microspheres with a diameter of 316.39??m ±?14.75??m and porous surface were prepared.The gelatine–modified PC(GEL–PC)microspheres exhibited better hydrophilicity and protein adsorption ability than PC microspheres.Enhancement of the adhesion and proliferation of the BMMSCs can be observed on GEL–PC microspheres after seven days.Conclusions:Therefore,this study demonstrates that employing a dynamic culture using GEL–PC microspheres is a promising method for deriving BMMSCs.It provides a new method to solve the problem of BMMSCs deficiency in clinical bone defect treatment.