The Role Of PI3K/Akt Signaling Pathway In Pinellia Ternata S Increasment Of The EPCs'activity And Its Anti-atherosclerosis

BackgroundThe cardiovascular and cerebrovascular diseases in the world are one of the main causes for human health threats.The cardiovascular and cerebrovascular diseases have the characteristics of high morbidity,high mortality and high recurrence.Atherosclerosis is one of the most common factors leading to cardiovascular and cerebrovascular diseases.Atherosclerosis begins with endothelial injury and dysfunction.Inflammation is an important factor in the formation of atherosclerosis.IL-6 plays an important role in the process of inflammation.Studies have found that Pinellia ternata can play an anti-inflammatory role by inhibiting the secretion of IL-6.PI3k/Akt/eNOS pathway plays a key role in mobilizing EPCs to differentiate into endothelial cells and improving EPCs function.This study was to explore whether Pinellia temata has anti-atherosclerosis effect and whether the anti-atherosclerosis effect of Pinellia ternata can be achieved through PI3K/Akt pathway.Purpose:?The therapeutic effect of Pinellia ternata on atherosclerotic SD rats was observed by HE staining.;? Flow cytometry was used to detect the number of EPCs in each group and the effect of Pinellia ternata on EPCs proliferation was analyzed.Whether Pinellia ternata enhanced the proliferation of EPCs through PI3K/Akt pathway was observed by comparison between Pinellia ternata treatment group and Pinellia ternata plus LY294002 group.The effect of Pinellia ternata on phosphorylation of VEGFR2 and eNOS was observed by quantitative detection of Western blot in each group.The possible mechanism of PI3K/Akt signaling pathway in the treatment of atherosclerosis by Pinellia ternata was analyzed;Method? 80 SPF-grade and 8-week-old male SD rats were used to establish the right common carotid atherosclerosis models;?60 SPF-grade 12-week-old SD rats were selected from the established atherosclerosis models and divided into 5 groups(13 rats in each group,all 12 weeks old):a)normal control group(normal SD rats without administration);b)Model group(SD rats with atherosclerosis);c)Pinellia ternata plus LY294002(PI3K/Akt specific inhibitor)group;d)Pinellia ternata treatment group;e)Pinellia ternata plus EPCs(EPCs cultured in vitro)group,HE staining was used to observe the thickness of intima and media,changes of calcification foci,elastic fibers,etc;(3)Flow cytometry was used to detect the quantity of EPCs in peripheral blood of normal control group,model group,Pinellia ternata plus LY294002 group,Pinellia ternata treatment group,Pinellia ternata plus EPCs group in 30 min,1 week,4 weeks and 8 weeks after intervention;?Western blot was used to detect the expression of VEGFR2,p-VEGFR2,eNOS,p-eNOS in the right common carotid artery tissue of the normal control group,model group,Pinellia ternata plus LY294002 group,Pinellia ternata treatment group,Pinellia ternata plus EPCs group in 30 min,1 week,4 weeks and 8 weeks after intervention;?Statistical analysis:All the experimental data in this experiment were analyzed by spss20.0.Graphpad prism5.0 software was used to map,and the related data were analyzed by multi-factor repeated measurement analysis of variance.The level of test was statistically significant(p<0.05).Results:? Pinellia ternata in the treatment of atherosclerosis is achieved by increasing the proliferation of EPCs through the PI3K/Akt pathway;?Pinellia ternata in the treatment of atherosclerosis is achieved by increasing the proliferation of endothelial progenitor cells,and the enhancement of endothelial progenitor cell migration by Pinellia ternata is achieved through PI3K/Akt pathway;?Pinellia ternata can promote the binding of VEGF to VEGFR2 on the membrane of EPCs,increase the phosphorylation of VEGFR2,phosphorylate eNOS through PI3K/Akt pathway,promote the mobilization,migration,homing of EPCs,and increase the proliferative activity of EPCs.ConclusionPinellia ternata has the effect of anti-atherosclerosis.Pinellia temata may enhance the proliferation of EPCs through PI3K/Akt pathway to act anti-atherosclerosis.The enhancement of proliferation activity may be caused by enhancing the binding of VEGF to VEGFR2 on the membrane of EPCs by Pinellia ternata,increasing the phosphorylation of VEGFR2 and eNOS through PI3K/Akt pathway,promoting the mobilization,migration,homing of EPCs,and increasing the proliferative activity of EPCs.