Curcumin Inhibits Proliferation And Migration Of Human Bladder Cancer T24 Cell Line By Modulating PI3K/AKT Signaling Pathway

Objective: To study the effect of curcumin on the proliferation and invasion of bladder cancer T24 cells and its mechanism,which will provede a basis for the development of new anti-bladder cancer drugs.Methods: The bladder cancer T24 cells were cultured and were treated with different concentrations(0,10,20,30 and 40 ?mol/L)of Curcumin to intervene 72 hours.The CCK8 assay was used to detect the inhibitory growth rate of the curcumin on T24 cells.The transwell matrix penetration assay was used to detect the cell migration capabilities.Apoptosis of T24 cells which treated by 20 or 40 ?mol/L curcumin were determined by flow cytometry analysis.The expressions of MMP2(matrix metalloproteinase),MMP9,AKT,p-AKT,PI3 K and p-PI3 K protein were analyzed by Western blotting.Resluts: CCK8 results show that curcumin can inhibit the proliferation of T24 cells in terms of concentration and time.Transwell results demonstrate that the intervention of curcumin can significantly reduce the migration of bladder cancer T24 cell and the inhibitory efficiency of the treatment of 10,20,30 and 40 ?mol/L curcumin was 27.4%,37.1%,58.1% and 64.5% respectively.The curcumin could induce apoptosis in T24 cells.Curcumin intervention can reduce the expressions of p-AKT,p-PI3 K,MMP2 and MMP9 in bladder cancer T24 cells in a dose-dependent manner.The higher the curcumin concentration was used,the lower expression of p-AKT,p-PI3 K,MMP2 and MMP9 were observed in T24 cells.Conclusion: The curcumin may down-regulate the expression of MMP2 and MMP9 by suppressing the PI3K/AKT signaling pathway,thereby inhibiting the proliferation and migration of bladder cancer T24 cell.