Micro Differentiation Of Blood Stasis Syndrome In Lupus Nephritis Based On MiR-127-3p And MiR-129-5p Molecules And Related Clinical Indicators

Objective From the perspective of miRNA and clinical indicators,this paper explores the micro differentiation of blood stasis syndrome in lupus nephritis,and provides stable objective indicators for clinical diagnosis and evaluation of curative effect.Methods A total of 60 patients with lupus nephritis who met the diagnostic criteria,inclusion criteria and exclusion criteria from the outpatient and inpatient nephrology department of our hospital were selected.They were divided into 30 patients with microscopic blood stasis syndrome and 30 patients with microscopic non-blood stasis syndrome.And a special person is responsible for filling in the "Case Information Form",recording the general information of the subject’s age,gender,and course of disease,performing RT-PCR testing on the subject,and measuring the expression of miR-127-3p,miR-129-5p And determine the relevant clinical indicators of the subject,including blood routine(WBC,LYM,RBC,HGB,PLT),renal function related indicators(BUN,Cr,UA,Cys-C),liver function related indicators(ALB,ALT,AST),inflammation-related indicators(CRP,ESR),immune function-related indicators(ANA spectrum,complement C3,complement C4),pathological indexes(AI,CI),lupus nephritis activity index(SLEDAI score).Further analysis of the correlation between the expression levels of miR-127-3p and miR-129-5p and the microscopic blood stasis syndrome in LN patients,and the analysis of related clinical indicators between the micro blood stasis syndrome group and the micro non-blood stasis syndrome group of lupus nephritis difference.Results Among the 60 patients with lupus nephritis,30 were in the micro blood stasis syndrome group,30 were in the micro non-blood stasis syndrome group,and the expression levels of miR-127-3p,miR-129-5p,BUN,Cr,UA,Cys-C,ESR,AI,CI,and SLEDAI points are higher than those in the micro non-blood stasis syndrome group(P<0.05),and the RBC,HGB,and PLT indicators in the micro blood stasis syndrome group are lower than those in the micro non blood stasis syndrome group.(P<0.05).There was no significant difference in WBC,LYM,ALB,ALT,AST,CRP,ANA spectrum,complement C3,and complement C4 between the two groups of patients(P>0.05).Conclusion 1.There was no significant difference in gender and age between the two groups of patients.2.The expression levels of miR-127-3p and miR-129-5p in the microscopic blood stasis syndrome group were significantly higher than those in the microscopic non-blood stasis syndrome group,indicating that these two molecules have the potential to serve as the microscopic basis for blood stasis syndrome.3.There were no significant differences in WBC,LYM,ALB,ALT,AST,CRP,ANA spectrum,complement C3,and complement C4 between the two groups of patients.4.The BUN,Cr,UA,Cys-C,ESR,AI,CI,and SLEDAI scores of the micro blood stasis syndrome group were higher than those of the micro non-blood stasis syndrome group.The microscopic non-blood stasis syndrome group is more serious.The microscopic blood stasis syndrome group has more severe inflammation damage than the microscopic non-blood stasis syndrome group.The microscopic blood stasis syndrome group has higher pathological activities and chronic kidney damage than the microscopic non-blood stasis syndrome group.The disease activity of patients with stasis syndrome group and microscopic blood stasis syndrome group was higher than that of microscopic non-blood stasis syndrome group.5.The RBC,HGB,and PLT indexes of the micro blood stasis syndrome group were lower than those of the micro non-blood stasis syndrome group,indicating that the patients in the micro blood stasis syndrome group had more severe anemia and had a greater risk of bleeding.In summary,the expression levels of miR-127-3p,miR-129-5p,RBC,HGB,PLT,BUN,Cr,UA,Cys-C,ESR,AI,CI,and SLEDAI scores may have some significance for clinical diagnosis of blood stasis syndrome in patients with LN.