Study On The Central Dynamic Expression Response Mechanism Of Electro-acupuncture In Rats With Gastric Ulcer Based On Metabolomic

Objective:To investigate the effects of electroacupuncture at Zusanli and Liangmen points on the repair of mucosal injury in rats with gastric ulcer(GU)and the response mechanism of central metabolism to the cerebral cortex and medullary tissue.In this study,1H-NMR metabolomics analysis of the cerebral cortex and medulla oblongata of gastric ulcer rats at different time points through electro-acupuncture intervention was combined with pathological morphological observation of gastric mucosa.Methods:According to the random number table method,54 male Sprague-Dawley(SD)rats were randomly divided into normal group(N group),model group(M group),and electro-acupuncture group(Group A).According to different intervention time,each group was further divided into 3 subgroups,namely 1d subgroup,4d subgroup,and 7d subgroup,with 6 in each subgroup.The rats in the model group and the electro-acupuncture group were given a gastric ulcer model induced by intragastric administration with 75%ethanol.After the modeling was completed,the rats in the electro-acupuncture group were intervened with electric acupuncture.After the intervention,the gastric mucosa tissue was taken to observe the dynamic expression changes of histopathology.And MRI metabolism was used to detect the dynamic expression of rat cerebral cortex and medulla metabolites.Results:Histopathological observation of the gastric mucosa of rats showed that the gastric mucosa of rats in the 1d and 4d subgroups of the model group showed cell necrosis,disordered glandular arrangement,and a large number of inflammatory cells infiltration,etc.At 7 days,the damage degree of the mucosa was improved.Electro-acupuncture did not improve much in the 1d subgroup,while the gastric mucosal damage in the 4d and 7d subgroups was continuously repaired.The result of~1 H-NMR metabolism results showed that 21 differential metabolites,19 metabolic pathways were found in the cerebral cortex and medulla of rats,respectively:1.Alanine,aspartate and glutamate metabolism,2.Glycine,serine and threonine metabolism,3.D-Glutamine and D-glutamate metabolism,4.Taurine and hypotaurine metabolism,5.Starch and sucrose metabolism,6.Glutathione metabolism7.Pyruvate metabolism,8.Aminoacyl-tRNA biosynthesis,9.Glyoxylate and dicarboxylate metabolism,10.Arginine biosynthesis,11.Arginine and proline metabolism,12.Glycolysis/Gluconeogenesis,13.Butanoate metabolism,14.Citrate cycle(TCA cycle),15.Primary bile acid biosynthesis,16.Galactose metabolism,17.Glycerophospholipid metabolism,18.Cysteine and methionine metabolism,19.Purine metabolism.Through induction,we further classified the metabolites contained in these metabolic pathways into energy-related metabolism,neurotransmitter related metabolism,cell and cell membrane related metabolism and antioxidant related metabolism according to their physiological functions.Conclusion:Acupuncture can effectively prevent and treat GU to a certain extent.After intervention for 4 days and 4 days,the degree of gastric mucosal damage continues to improve,and the treatment effect is stable.It is found in tissue metabolites that gastric ulcer can prevent and protect cell membrane damage by some relate pathways of the human central nervous system.Electro-acupuncture intervention for 4 days is a more obvious turning point.After 7 days of electro-acupuncture intervention,the callback effect of metabolite content has stabilized.Therefore,this study indicates that electro-acupuncture intervention for GU lasts for at least four days and more,and mainly affects the metabolism of the central nervous system to play a role in prevention and treatment.The response mechanism of other related biochemical indicators and more detection methods of analysis needs further research.