Effect Of Serum Of Yangjing Zhongyu Decoction On Maturation Of Mouse Oocytes In Vitro

ObjecktiveTo investigate the relationship between Serum of containing Yangjing Zhongyu Decoction in promoting mouse oocyte maturation in vitro and VEGF-A,cdc2,PDGFA and PDGFR?.MethodsBest blood collection time point after gavage was monitored by High performance liquid chromatography,then to prepare serum.Immature oocyte-granulos cell complexes were collected and divided into blank group,blank serum group,Yangjing Zhongyu Decoction serum group,FSH group.The maturation rate,fertilization rate and cleavage rate of mouse oocytes were calculated after in vitro culture.Immunofluorescence and Real-Time PCR were used to detect the protein and mRNA expression of VEGF-A and cdc2 in oocytes.Western blot and Real-Time PCR were used to detect the protein and mRNA expression of PDGFA and PDGFR? in oocytes.ResultsThe serum was monitored by High performance liquid chromatograpHy,and 0.5 h was the highest drug absorption peak after gavage Yangjing Zhongyu Decoction.Compared with the blank group and blank serum group,the Yangjing Zhongyu Decoction serum group and FSH group could significantly increase the oocyte maturation rate and fertilization rate in vitro(P<0.05 or P<0.01).Compared with the blank group and blank serum group,the Yangjing Zhongyu Decoction serum group and FSH group of expression level of VEGF-A,PDGFA,PDGFR? protein and mRNA were down-regulate(P<0.05 or P<0.01);the expression of cdc2 protein and mRNA Level was up(P<0.05 or P<0.01)ConclusionSerum of containing Yangjing Zhongyu Decoction may promotes the in vitro maturation of mouse oocytes,the mechanism may related to down-regulation VEGF-A,PDGFA,PDGFR?,up-regulation of cdc2 protein and mRNA expression.