| ObjectiveThis study aims to investigate the distribution of non-bacterial pathogens in the patients with ARI in Nanchang of Jiangxi province and to identify the association between ARI pathogens and clinical symptoms,diagnosis,gender,age,and infection site further.MethodsThe nasopharyngeal exfoliated cells and secretions were collected with throat swabs in the adult who suffered from ARI and visited the Department for High Fever of the First Affiliated Hospital of Nanchang University from December 1,2019,to January 21,2020.Application of multiplex RT-PCR combined with capillary electrophoresis to detect 13 common non-bacterial respiratory pathogens namely total Influenza Virus type A(InfA)(H1N1(2009),H3N2,H7N9 and H5N2),H1N1(2009),H3N2,Human Respiratory Syncytial Virus(HRSV),Influenza Virus type B(InfB),Human Parainfluenza Virus(HPIV),Human Adenovirus(HADV),Human Rhinovirus(HRV),Bocavirus(Boca),Human Coronavirus(HCOV),Human Metapneumovirus(HMPV),Mycoplasma pneumonia(MP)and Chlamydia(Ch).The medical history and clinical data of these patients were collected for statistical analysis.We used the SPSS 25.0 software to analyze all statistical data,with ?=0.05 as the test level.The count data was described in the form of the number of cases or rates.Result(1)Positive detection of ARI non-bacterial pathogens: The total positive detection rate of ARI pathogens across 119 patients is 69.75%(83/119),and 69 cases are single pathogen infection,accounting for 83.13%(69/83)of the positive cases.Influenza Virus type A(total InfA,H1N1(2009),H3N2)(29/119,24.37%),InfB(22/119,18.49%),HRV(6/119,5.04%),HMPV(4/119,3.36%),HADV(3/119,2.52%),HRSV(3/119,2.52%)and HPIV(2/119,1.68%).Fourteen cases are co-infection,accounting for 16.87%(14/83)of the positive cases,which are all double-pathogen infection.(2)Positive detection of pathogens with different clinical symptoms: The positive rate of pathogens increased significantly at T?39.2?(p=0.01).The T?38? accompanied by cough,runny nose,sore throat,headache,or muscle aches will increase the positive rate of pathogens significantly(p?0.05),while the T?38? accompanied by sputum,chest tightness,or chest pain will not(p?0.05).(3)The relationship between ARI pathogen and age and gender: The result shows that no significant association between the different genders groups in the positive detection rate of pathogens.However,the positive rate of pathogens in the 49-82 age group is higher than that in the 16-49 age group(p?0.05).(4)The relationship between ARI pathogens,infection sites and diagnosis: the positive detection rate of ARI pathogens in the acute upper respiratory infection(AURI)is 61.34%(73/119),while the positive detection rate in the acute lower respiratory infection(ALRI)is 8.40%(10/119),and the difference between AURI and ALRI is significant(p?0.01).The positive rate of single pathogen infection in AURI is significantly higher than ALRI(p?0.01)but the positive rate of double-pathogen infection is the opposite(p?0.01).Besides,the acute viral pharyngitis or laryngitis have the highest pathogen positive rate,and the difference between the five AURI diseases is significant as well(p=0.04).(5)The relationship between ARI pathogens in the single pathogen infection and the co-infection: The Influenza Virus type A and InfB are both the most detected pathogen in a single infection and the co-infection(45/69,65.2%;17/28,60.71%).No significant association of thirteen pathogens between the single infection and the co-infection is observed(p?0.05).Conclusion(1)In the Nanchang erea of Jiangxi province,the overall positive rate of non-bacterial pathogens in outpatient ARI patients is 69.75%.The positive rate of single pathogen infection is 57.98%,and the positive rate of Influenza Virus type A is the highest.Besides,the positive rate of co-infection is 11.76%,which is dominated by double-pathogen infection.(2)When the patient develops fever accompanied by cough,runny nose,sore throat,headache,or muscle aches,the positive rate of pathogens will increase significantly.(3)The overall positive rate of ARI pathogens inpatients is significantly related to infection sites and age but not gender,that is,the positive rate of pathogens in 49-82 age group is higher than that in the 16-49 age group and AURI is higher than ALRI.The positive rate of single pathogen infection in AURI is higher than in ALRI but the positive rate of double-pathogen infection is the opposite.In addition,the difference between the positive pathogen rate among the five AURI diseases is significant as well.(4)There is no relationship of thirteen pathogens between the single infection and the co-infection. |
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