| Objective:The purpose of this study is to reveal the effects of exercise and acupuncture alone or in combination on blood lipids and serum inflammatory factors in obese rats through animal experiments,and to preliminarily explore the differences in the effects of different intervention methods on obese rats.Methods:100 male SD rats aged 5 weeks were randomly divided into 2 groups: control group(C,n=10)and model group(M,n=90).The control group was fed normal diet,and the model group was fed high-fat diet to establish the obesity model.After eight weeks,with weight more than ordinary feed group of rats in model group average weight + 1.4 times the standard deviation of rats as obesity standards,building success only 39,randomly selected 32 only divided into 4 groups,obese control group(OC,n = 8),obesity group(OE,n = 8),obesity acupuncture group(OA),n = 8),obesity plus acupuncture group(OEA,n =8).Aerobic exercise intervention was carried out in the OE group and OEA group for 4weeks.In the OA group and OEA,acupuncture and moxibustion intervention was carried out for 4 weeks.During the intervention,the body mass of the rats was measured once a week,and their daily food intake was recorded.After the experiment,the rats in each group fasted overnight,the heart blood was taken after chloral hydrate anesthesia.The contents of T-CHO,TG,HDL-C and LDL-C in serum were determined by enzymatic method,and the contents of IL-6,IL-10 and MCP-1 in serum were determined by enzyme-linked immunoassay.Results :1.Body mass(1)After 8 weeks of modeling,compared with group C,the body mass of rats in group M was significantly increased(P<0.05);(2)After 4 weeks of intervention,compared with OC group,the body mass of rats in OE group,OA group and OEA group was significantly reduced(P<0.05).2.Food intake(1)During the modeling period,compared with group C,the food intake of rats in group M at 1-8 weeks was significantly reduced(P<0.05);(2)During the intervention period at week 4,compared with the OC group,the amount of food intake at week 1 to 4 in the OE group was significantly increased(P<0.05);the amount of food intake at week 2 in the OA group was significantly decreased(P<0.05),and there was no significant difference in other weeks(P>0.05).There was also no significant difference about the amount of food intake at week 1 to 4 in the OEA group(P>0.05).Compared with the OE group,the food intake in the OA group and the OEA group was significantly reduced from week 1 to week 4(P<0.05);Compared with OA group,OEA group had significantly increased food intake at week 2(P < 0.05)?significantly decreased food intake at week 4(P<0.05)? and there was no significant difference in other weeks.3.Lee's index(1)It was significantly higher in OC group than in NC group(P<0.05);(2)After 4 weeks of intervention,compared with OC group,Lee's index of rats in OE group,OA group and OEA group was significantly decreased(P<0.05)..4.Serum T-CHO,TG,HDL-C,and LDL-C(1)Compared with NC group,T-CHO?TG?LDL-C contents significantly increased,and HDL-C content significantly decreased in OC group(P<0.05).(2)After 4 weeks of intervention,compared with OC group,T-CHO and TG content were no significant difference(P>0.05)and HDL-C content significantly increased(P < 0.05),LDL-C content decreased significantly(P < 0.05)in OE group,;T-CHO content in OA group,there was no significant difference(P > 0.05),TG content decreased significantly(P < 0.05),HDL-C content significantly increased(P < 0.05),LDL-C content decreased significantly(P<0.05).The contents of T-CHO,TG and LDL-C in OEA group significantly decreased(P<0.05),and the content of HDL-C significantly increased(P>0.05).(3)After 4 weeks of intervention,compared with OE group,T-CHO content had nosignificant difference(P>0.05),TG content significantly reduced(P<0.05),HDL-C content had no significant difference(P>0.05)and LDL-C content significantly reduced(P<0.05)in OA and OEA group.(4)After 4 weeks of intervention,compared with the OA group,the LDL-C content of rats in the OEA group was significantly decreased(P<0.05),T-CHO?TG and HDL-C content had no significant difference(P>0.05).5.Serum levels of IL-6,IL-10 and MCP-1(1)They were significantly higher in the OC group than in the NC group(P<0.05).(2)After 4 weeks of intervention,compared with OC group,the contents of IL-6 and MCP-1 in OE group,OA group and OEA group were significantly reduced(P<0.05);IL-10 had no significant difference(P>0.05).(3)After 4 weeks of intervention,compared with the OE group,the contents of IL-6 and IL-10 in OA group and OEA group showed no significant difference(P>0.05),and the contents of MCP-1 were significantly reduced(P<0.05).(4)After 4 weeks of intervention,compared with the OA group,the contents of IL-6?IL-10 and MCP-1 in OEA group had no significant difference(P>0.05).Conclusion :1.High-fat diet causes the increase of body mass and Lee's index in rats.Both the single and combined intervention of exercise and acupuncture can reduce the body mass and Lee's index in obese rats.2.High-fat diet causes dyslipidemia,and both exercise and acupuncture alone or in combination can improve dyslipidemia in obese rats,but the effect of exercise plus acupuncture is better than that of exercise or acupuncture alone.3.High-fat diet causes elevated levels of serum inflammatory factors,and both exercise and acupuncture alone or in combination can improve the chronic inflammatory state of obese rats. |
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