| Since 2013,the research group has successively carried out the fermentation of the cow manure and corn cob as a fermentation substrate,and used the secondary fermentation method to prepare the fermented material for the cultivation of the Agaricus blazei.The research results showed that the method could obtain the cultivation material suitable for the growth of the Agaricus blazei.The rationale for second fermentation of cow dung and corn cob was presumed to be based on the microbial flora in cow dung on degrade the lignin and cellulose degradation of corn cob,thus obtaining the carbon and nitrogen sources suitable for the growth of Agaricus blazei.However,the secondary fermentation process takes a long time and the fermentation temperature is high.Our previous research team also found that the modified strain of Neurospora has good degradation effect on corn stover.Therefore,this project selected qa-myc-his-NCU07340 strain to explore its synergistic effect of this strain with cow dung on fermenting corn cob,which would provide data for exploration of compound mirobrobes on fermentation of cob for edible fungi cultivation.In this project,corn cob and cow dung were mixed and fermented according to the method used in our lab.The PDA medium was used to activate Neurospora crassa,which was then mixed with corn stover without or with cow dung.The pH and conductivity of the fermented material were measured during the fermentation.Moisture content,total nitrogen,humic acid,cellulose and lignin content were also measured at the end of fermentation.Then the structural characterization and the polysaccharide components of the corn cob fermentation material was studied.Finally the mushroom was cultivated to determine the best fermentation formula.During the fermentation process,the pH of the corncobs treated was neutral or weakly alkaline,which was suitable for the growth of edible fungi.The total nitrogen content of Neurospora crassa 7340 treated corn cobs was 37.22±0.44 g/kg,slightly higher than the corn cob treated by cow dung(36.77±0.41 g/kg).The common indicators of other edible fungi fermentation materials were comparable with those fermented with cow dung.The strain Neurospora crassa 7340 had a strong degradation effect on lignin in corn cob,which can further promote the degradation of cellulose.The corncob cellulose content was 25.23±0.39 %,and the cellulose content was the lowest in each treated corn cob,indicating stronger cellulose degradation ability than other groups and the synergistic effect of cow dung and qa-myc-his-NCU07340 strain on the unfermented corn cob.The crystallinity was 66.7% when the corn cob was fermented at 37 ? for 2 d and at 57 ? for another 3 d,the crystallinity was 62.6 %.While the crystallinity was 51.1% of the corn cob fermented with cow dung and qa-5myc-6his7340,indicating that the strain NCU07340 destroyed the cellulose in the corn cob further.In the soil-covering experiments,the fermented corn cob with in the corn manure alone grew to 2/3 of the mushroom pack at 19 d,and the qa-myc-his-NCU07340 strain co-fermented with the cow dung to grow the mycelium of the mushroom in the corn cob at 14 d.In summary,the combination of qa-myc-his-NCU07340 with cow dung showed better degradation effects on the cellulose and other components in the corn cob,which showed improved growth of the Brazilian mushroom. |
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