| Chronic renal failure(CRF)is a complicated disease with slow progress,diverse complications,difficult treatment,and expensive medical expenses.It has become a common disorder that endangers people's wellness.Therefore,increasing research on chronic renal failure and enriching treatments are of great significance for delaying the progression of the disease and improving the quality of life of patients.Suspended acupuncture therapy is a unique treatment that combines traditional Chinese medicine qigong and acupuncture to create a unique treatment.It has advantages of painless,non-invasive and not subject to forbidden or caution limits.In this study,we stimulate "Shenshu" with suspended acupuncture as a treatment.The effect of suspended acupuncture therapy on rats,kidney function,anemia and renal interstitial fibrosis related factors was observed.The prevention and treatment of suspended acupuncture therapy on chronic renal failure were discussed.ObjectiveIn this experiment,the rats with chronic renal failure established by intragastric administration of adenine were used as the research object.Uremic clearance granule(UCG)were used as the positive control group,and the "shenshu" was used as the treatment acupoint.The rats were treated with suspended acupuncture therapy at the beginning of modeling.Genes and minerals such as RBC,HCT,Hb,PLT,P3+,Ca2+,Scr,BUN,TGF-?1,Smad7 and the effects of morphological changes in rat kidney tissue were observed to investigate the prevention and treatment of chronic renal failure by suspended acupuncture therapy and exploring the possible mechanism of suspended acupuncture therapy to delay the progression of renal interstitial fibrosis.Method40 male SD rats were randomly divided into normal group,model group,medication group and suspended acupuncture group,with 10 rats in each group.The adenine was mixed with distilled water to prepare a 0.25%adenine suspension,and the rats except the normal group were intragastrically administered at a dose of 200 mg/kg/d for 28 consecutive days;The medication group was intragastrically administrated with adenine suspension every morning,the UCG were dissolved in distilled water to prepare a 2.25%solution,and the medication group was administered at a dose of 2.2 g/kg/d every afternoon,the other groups were given the same amount of distilled water;The suspended acupuncture group was intragastrically administrated with adenine suspension every morning,and administered with distilled water at the same dose of medication group every afternoon,and the rats was treated with suspended acupuncture for 30 minutes.The body weight of each group was weighed every week.After the experiment,the genes and minerals such as RBC,HCT,Hb,PLT,P3+,Ca2+,Scr,BUN were detected by automatic biochemical analyzer,expression of TGF-?1 and Smad7 were detected in rat kidney by immunohistochemistry,and we weighed kidney wet weight,renal tissue-was subjected to HE staining and Masson staining and pathological changes were observed.Results1 Body weight and kidney weight of rats:From the 2nd week,the body weight of the rats in the model group,the medication group and the suspended acupuncture group was significantly different from that in the normal group(P<0.05).The weight of the rats in the suspended acupuncture group was significantly increased than the model group except the 3rd week(P<0.05).Compared with the normal group,kidney to body weight ratio of the model group was significantly increased(P<0.05),and of the suspended acupuncture group was lower than the model group(P<0.05).There was no significant difference between the suspended acupuncture group and the medication group(P>0.05).2 RBC,HCT,Hb in whole blood:Compared with the normal group,the RBC,Hb and HCT contents in the model group were significantly decreased(P<0.05).Compared with the model group,the contents of RBC,Hb and HCT in the suspended acupuncture group were significantly increased(P<0.05).There was no significant difference between the suspended acupuncture group and the medication group(P>0.05).3 P3+ and Ca2+ in Serum:Compared with the normal group,the serum P3+ content of the model group was significantly increased(P<0.05),and the Ca2+ content was significantly decreased(P<0.05).The P3+ content of the rats in the suspended acupuncture group was significantly decreased(P<0.05),and the Ca2+ content was significantly increased(P<0.05).There was no significant difference between the suspended acupuncture group and the medication group(P>0.05).4 The expression of TGF-?1:Compared with the normal group,the AOD was significantly increased in the renal tissue of the model group(P<0.01).Compared with the model group,the expression of TGF-?1 in the suspended acupuncture group was significantly decreased(P<0.01).There was no significant difference between the suspended acupuncture group and the medication group(P>0.05).5 Scr and Bun in serum:Compared with the normal group,the serum Scr and Bun levels in the model group were increased(P<0.05),and the Scr and Bun in the suspended acupuncture group were significantly decreased(P<0.05).There was no significant difference between the suspended acupuncture group and the medication group(P>0.05).6 HE staining section:In the normal group,the kidney structure was intact,the glomerulus was not atrophied,and the renal tubules and collecting ducts were not dilated or atrophied,and there was no fibrous tissue hyperplasia and inflammatory cell infiltration.Renal tubule yellow-green crystal deposition and granuloma formation,tubular dilatation,tubular degeneration/necrosis,interstitial cell infiltration and interstitial fibrosis in the model group.There was no significant reduction in the degree of renal granuloma formation in the medication group,and the other lesions showed a slight decrease trend.Renal tubular dilatation,renal tubular degeneration/necrosis and other lesions in the suspended acupuncture group were all relieved.5 Masson staining section:In the normal group,the collagen fibers in the normal group were stained with dark blue around the blood vessels,and there was no obvious blue coloration in the tissues.In the model group,except for the collagen fibers around the blood vessels,dark blue staining was observed.A wide range of renal interstitial broadening was observed,which showed a pale blue coloration.This is a special staining of renal interstitial fibrosis,in which dark blue scattered in focal hyperplasia coloring collagen fibers.The degree of hyperplasia of focal collagen fibers in the medication group was slightly reduced,and the degree of renal fibrosis has a tendency to decrease.The degree of renal fibrosis in the suspended acupuncture group was slightly reduced,and the degree of hyperplasia of focal collagen fibers has a tendency to decrease.Conclusion1 Suspended acupuncture therapy has a certain protective effect on the kidney of rats with modeling.First,uspended acupuncture therapy can increase the speed and amplitude of weight gain in model rats.Second,suspended acupuncture therapy can reduce the kidney to body weight ratio of model rats and reduce the kidney hypertrophy index.Third,it can be seen by pathological section,that suspended acupuncture therapy can improve the pathological morphology of kidney in model rats,reduce renal tubular deposition,tubular dilatation,tubular degeneration/necrosis and renal interstitial fibrosis.2 Suspended acupuncture therapy can effectively increase the content of RBC,HCT,Hb,which suggest that the suspended acupuncture therapy can improve renal anemia,and improve blood supply to the kidney.3 Suspended acupucture therapy cam effectively reduce the content of P3+ and increase the content of Ca2+,adjust the state of high phosphorus and low calcium,improve the metabolism of calcium and phosphorus,and reduce the risk of complications such as renal bone disease and cardiovascular disease.4 Suspended acupuncture therapy can effectively reduce the expression of TGF-?1 in the kidney,reduce the deposition of extracellular matrix,and delay the renal interstitial fibrosis.5 Suspended acupuncture therapy can reduce the content of Scr and BUN,and enhance kidney function.6 Suspended acupuncture therapy has a certain degree of prevention and treatment for chronic renal failure.The mechanism may be to adjust the renal microcirculation by improving the supply of local blood in the tubules and interstitial,improve renal microcirculation,improve calcium and phosphorus metabolism.To decrease the expression of TGF-?1 in the kidney.Reducing renal interstitial fibrosis,so that the increase of blood oxygen supply and the reduction of interstitial fibrosis form a virtuous circle,and ultimately enhance kidney function and improve the quality of life of patients. |
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