| With the gradual improvement of the international status of traditional Chinese medicine(TCM),the standardization of TCM has become the inevitable trend.The standardization of TCM refers to the standardization of quality,and the selection of quality control components is an important part of the standardization of TCM,which is related to the development of TCM and national strategic issues.In the current Chinese Pharmacopoeia(2015 Edition),the quality of TCM is evaluated by the content of one or several index components.However,many components are lack of specificity and biological activity,which is of little significance to the quality control of TCM.For the quality control components of TCM,the most important thing is to be related to the efficacy.Only by finding the effective components related to the efficacy and activity,and establishing the quality evaluation method that can reflect the efficacy,and the quality of TCM can be fundamentally controlled.In recent years,studies have shown that not all components of the drug can play a role in its efficacy after oral administration.Only the components that ultimately enter the blood can play a role in its efficacy.With the development of network informatics,network pharmacology has become a new way to explain the therapeutic mechanism of TCM.Therefore,in order to improve the quality standard of TCM,this study,taking Chrysanthemum morifolium Ramat.,Gentianae Macrophyllae Radix,and Lycium barbarum L.as examples,attempt to establish a set of systematic,scientific and feasible method for the selection of quality control components of TCM by using the mode of "chemical compositions-blood compositions-network targets".ObjectiveTo establish a new method of screening the quality control components of traditional Chinese medicine by the mode of "chemical component,blood component and network target".Method1.Chemical compositions analysis of Chrysanthemum morifolium Ramat.,Gentiana macrophylla Radix,and Lycium barbarum L.:the chemical compositions of three kinds of TCM were studied preliminarily by HPLC,and the known chemical compositions of the TCM were determined preliminarily by comparison with the reference substances;the chemical compositions of the three kinds of TCM were identified by UPLC-LTQ-Orbitrap-HRMS technology through the first-order mass spectrometry data,second-order fragment ion,reference substances,references and databases.2.Study on the multi-component metabolism of Chrysanthemum morifolium Ramat.,Gentiana straminea Maxim.,and Lycium barbarum L:in this study,rats were taken as the experimental objects,and the water extracts of three kinds of medicinal materials were taken as the experimental drugs.After the treatment of gastric perfusion and intestinal perfusion,the plasma samples of rats were collected in the abdominal aorta,mesenteric vein and femoral vein respectively.After the plasma samples were treated with SPE column,the plasma samples were also detected by HPLC and UPLC-LTQ-Orbitrap-HRMS to find the components of the original blood,and to study the metabolism process and results of the drugs in different organs of rats.3.Study on the selection of quality control components in Chrysanthemum morifolium Ramat.,Gentiana straminea Maxim.,and Lycium barbarum L:collect the targets of blood chemical components and disease targets by network pharmacology method,select the eligible core targets according to topological parameters,bring the core targets into David database for KEGG pathway analysis,and further find the disease-related pathway and the important targets on t pathway,the compounds with interaction relationship between important targets were screened as quality control components,and the potentially mechanism of these components in the treatment of diseases was clarified.Results1.54 chemical components were identified by LC-MS analysis of Chrysanthemum morifolium Ramat.most of which were flavonoids.33 chemical components were identified by LC-MS analysis of Gentiana straminea Maxim.including most of which were iridoid terpenes.The water extract of Lycium barbarum L.was analyzed by LC-MS,and 33 chemical components were identified,most of which were alkaloids,organic acids,and flavonoids compounds.2.After HPLC and LC-MS analysis of each plasma sample and comparison with water extract,it is determined that there are 14 primordial blood components in Chrysanthemum morifolium Ramat.,most of which are flavonoids;there are also 14 primordial blood components in Gentiana straminea Maxim.,most of which are iridoid terpenoids;there are 20 primordial blood components in Lycium barbarum L3.Based on the analysis of network pharmacology,12 primordial components including caffeic acid,chrysophanol,luteolin,asiatica,apigenin,artichoke,acacia,apigenin-7-o-glucoside,apigenin-7-o-glucuronide,6-hydroxyluteolin-7-glucoside,luteolin-7-o-glucuronide and acacia-7-o-glucuronide were identified as Chrysanthemum morifolium Ramat..The quality control components of gentiopicroside,isovitexin,icariin and oleanolic acid were used as the quality control components of Gentiana straminea Maxim.,and catechin,chlorogenic acid,ferulic acid,rutin and salicylic acid were used as the quality control components of Lycium barbarum LConclusionIn this study,the quality control components of Chrysanthemum morifolium Ramat.,Gentiana,straminea Maxim.,Lycium barbarum L.were screened,and the potentially mechanisms of their treatment were clarified.It is proved that the model of "chemical composition-blood composition-network target" is simple and feasible for the selection of quality control components of TCM,which can provide reference for other researches. |
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