Effects Of AM Fungus And Hormone GR24 On The Growth And Development Of Traditional Chinese Salvia Miltiorrhiza And Atractylodes Macrocephala

Arbuscular mycorrhizal fungus can symbiotically grow with the roots of most plant species to form mycorrhizas.As an obligate parasitic endophytic fungus,AM fungus absorbs mineral nutrients from the soil through extra-root hyphae,performs a series of assimilation,and then transfers to the root system of the host plant,at the same time,the plant will return carbohydrates to support the growth and development of AM fungus.This mutually beneficial symbiosis mechanism is regulated by both parties and related nutrients.For example,the type and concentration of nitrogen and phosphorus will affect this mechanism.As a microbial fertilizer,AM fungus has replaced chemical fertilizers and has been widely used in the agricultural product industry.At present,research in the field of medicinal materials is also popular.Studies have shown that the symbiosis of AM fungi on medicinal plants can produce positive effects,but it is not clear whether it can improve the medicinal components of medicinal plants.The synthetic strigolactone structural analogue GR24 has been shown to promote the identification and germination of AM fungal spores,but it is not relevant whether it can promote the infection of medicinal plants and AM fungi and whether it can promote the transportation and accumulation of nutrients the study.In this paper,the Chinese medicinal plants Salvia miltiorrhiza and Atractylodes macro-cephala as the research objects,by exploring the effect of exogenous hormone GR24on the growth and development of salvia and atractylodes seedlings,and the effect of exogenous hormone GR24 and different nitrogen sources on the accumulation of nutrients in salvia and atractylodes,it lays the foundation for researching medicinal plant bacterial fertilizer and improving the quality and quality of medicinal plant.The experimental content and results of this research are as follows:Through the exogenous hormone strigolactone GR24,the seedlings of salvia and atractylodes were treated at different times,explore the effects of GR24 treatment on AM fungal infection and host plants under different time.Experimental results show,the hormone GR24 at a concentration of 1/10,000 can significantly promote the AM fungus infection on the 30th day.With the increase of the treatment time,GR24 accumulates,and there is no significant difference in the infection rate of AM fungi.It is proved that with the accumulation of the hormone GR24,the promotion effect on the infection rate of AM fungi is reduced,and in the seedling stage,GR24 has no significant effect on the biomass of salvia and atractylodes.By infecting salvia plants,and then treated with different nitrogen sources and hormone GR24,explore the effects of hormone GR24 and AM fungi on the growth and development of salvia under different nitrogen sources.Experimental results show,it can be seen that under different nitrogen sources,O processing can significantly promote the infection rate of AM fungi,and it has a significant promoting effect on the biomass of Salvia miltiorrhiza.AM fungus have significant effects on the accumulation of nitrogen and phosphorus in the shoots of salvia in individual nitrogen source groups.Among them,the O processing promotes the accumulation of above-ground phosphorus,and U processing promotes the accumulation of above-ground nitrogen.Infection of AM fungi significantly promotes the accumulation of nitrogen and phosphorus underground.The content of arginine is highest in the AM+J group,which proves that AM fungi can promote the accumulation of mycorrhizal arginine.AM fungus treatment contributes to the accumulation of total sugars in salvia.Hormone GR24 has no significant effect on the nitrogen and phosphorus content and arginine content in the upper and lower parts of the ground,it proves that strigolactone GR24 as a hormone may be involved in the recognition of AM fungi and the germination of spores,but it does not participate in the nutritional conversion of the two or has a small impact on the nutritional conversion.Through the effects of different nitrogen sources and GR24 on atractylodes,it can be seen that AM fungi can promote the biomass of atractylodes to a certain extent,which is significant when the nitrogen source is O processing.The effect of the hormone GR24 on the AM infection rate was significant in the O processing.According to the experimental results,O processing is beneficial to the accumulation of total phosphorus in the ground under the AM fungus infection,while U processing is beneficial to the accumulation of total nitrogen in the ground,and the hormone GR24 has a positive effect on AM fungi.Atractylone is the main component of the volatile oil of Atractylodes Rhizome.After testing,AM fungus has a positive effect on the accumulation of atractylone content,but the hormone GR24 has no significant effect on the accumulation of atractylone content.Through three-chamber culture of salvia plants,different forms of nitrogen sources were added to the mycelium chamber to explore the best nitrogen source for nutrient exchange between Extraradical hyphae（ERM）and Intraradical hyphaer（IRM）.The experimental results show that ammonium chloride is more conducive to the growth of the roots of salvia,while potassium nitrate significantly promotes the growth of the shoots of salvia.When the nitrogen source is ammonium chloride,the AM fungus infection rate is significant.And under AM fungus infection,the ammonium chloride treatment group significantly promoted the accumulation of total nitrogen,total phosphorus and the number of spores in the hyphae compartment of Salvia miltiorrhiza.Arginine content also reached significance in the AM+ammonium chloride group.The results show that the NH₄⁺absorption capacity of the extra-root hyphae is stronger than other N sources（organic nitrogen sources,NO₃^-,amino acids）,which is more conducive to the infection of AM fungi.Moreover,AM fungi has a significantly higher assimilation effect on ammonium root ions than other groups,so it is more conducive to the growth and development of salvia plants.