Study On Serum Il-17,IL-6 And TNF-α Levels In Patients With Diabetic Retinopathy

ObjectiveTo observe the serum levels of interleukin-17(IL-17),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in patients with diabetic retinopathy(DR),and to analyze the correlation among serum IL-17,IL-6,TNF-α and DR.MethodsAll 49 diabetic retinopathy patients in Jiangsu Taizhou People’s Hospital were consecutively enrolled from April to January,2020.They were divided into two groups according to the grading standard of diabetic retinopathy,that is,21 patients in the proliferative diabetic retinopathy group(PDR group),28 patients in the non-proliferative diabetic retinopathy group(NPDR group).Another 26 people treated for other eye diseases in our hospital during the same period were selected as the normal control group(NC group).General clinical data of the selected patients were collected,including age,gender,body mass index(BMI),systolic blood pressure(SBP),diastolic blood pressure(DBP),diabetes duration and hypertension duration.Serum from the selected patients were collected.Serum fasting blood-glucose(FBG),creatinine(Cr),and blood urea nitrogen(BUN)were all detected.In addition,glomerular filtration rate(GFR)of all patients were evaluated.Enzyme-linked immune sorbent assay(ELISA)was used to detect the serum IL-17,IL-6,and TNF-αlevels.Spearman analysis was used to determine the relationship among IL-17,IL-6,TNF-α and SBP,FBG,Cr,BUN,GFR.Logistic regression analysis was used to determine the effect of factors.Results1.Compared with the NC group,the levels of SBP,FBG,Cr,BUN,IL-17,IL-6,TNF-α were significantly increased and the level of GFR was significantly decreased in PDR group(P <0.05).The levels of FBG,Cr,BUN,IL-17,IL-6,and TNF-α were significantly increased(P <0.05)in NPDR group when compared with NC group,but there was no significant difference in GFR levels(P > 0.05).There were no significant differences in SBP,diabetes duration,hypertension duration,FBG,Cr,BUN,GFR,IL-17,IL-6 and TNF-α between PDR and NPDR group(P > 0.05).The clinical data including age,BMI,DBP of PDR,NPDR and NC groups did not differ significantly(P > 0.05).2.The serum IL-17 levels of DR patients were significantly positive correlated with Cr and BUN levels(r = 0.296,P = 0.039;r = 0.527,P <0.001),and were significantly negative correlated with GFR levels(r =-0.388,P = 0.006),however,the correlation with FBG levels did not reach statistical significance(r = 0.167,P =0.251).In DR patients,significant positive correlations were also observed between serum IL-6 levels and FBG,Cr,BUN levels(r = 0.314,P = 0.028;r = 0.380,P =0.007;r = 0.481,P <0.001),but not GFR levels(r =-0.493,P <0.001).Moreover,the serum TNF-α levels were significantly positive correlated with Cr,BUN levels(r =0.634,P <0.001;r = 0.680,P <0.001),and significantly negative correlated with GFR levels(r =-0.870,P <0.001)in DR patients.Furthermore,the serum TNF-α levels did not correlate with FBG level in DR patients(r =-0.058,P = 0.693).3.Logistic regression analysis showed that IL-6 was an independent risk factor for DR(P < 0.05).ConclusionSerum IL-17,IL-6,and TNF-α levels are significantly increased in DR patients,which may be risk factors for the development and progression of DR.