Effect Of Total Thyroidectomy On MRNA Expression Of VEGF,sFas And SFasL In Thyroid Cancer Patients

ObjectiveTo investigate the effect of total thyroidectomy on the expression of VEGF,sFas and sFas L m RNA in patients with thyroid cancer,and the correlation of tumor related inflammatory factors IL-6,IL-8 and TNF-α,so as to explore the mechanism of total thyroidectomy on patients with thyroid cancer.Methods60 patients with thyroid cancer who underwent total thyroidectomy in our hospital from Dec.2017 to Dec.2020 were selected as the research objects by prospective case-control study.At the same time,the expression levels of VEGF,sFas and sFas L m RNA,inflammatory factors(serum IL-6,IL-8)and TNF-α were recorded before operation and 30 days after operation.The levels of VEGF,sFas,sFas L in blood samples before operation and 30 days after operation were compared m RNA expression,serum IL-6,IL-8,TNF-α levels and their correlation.Results1.The difference of the positive expression of VEGF(t = 62.225,P = 0.000),sFas(t = 31.113,P = 0.000)and sFas L(t= 31.113,P= 0.000)in thyroid cancer tissue was statistical y significant before and after total thyroidectomy,and the positive expression level of VEGF,sFas and sFas L after total thyroidectomy was lower than that before total thyroidectomy.2.The difference of the expression levels of VEGF,sFas,sFas L,IL-6,IL-8 and TNF-α in different clinicopathological features of thyroid cancer before and after total thyroidectomy was statistical y significant(P < 0.05).3.The difference of the expression levels of VEGF,sFas,sFas L in < 55 years old and ≥ 55 years old,clinical type of thyroid tumor,metastasis or not,and tumor location before total thyroidectomy except for different gender was statistical y significant(P < 0.05).The difference of the expression levels of IL-6 and IL-8 in <55 years old and ≥ 55 years old,clinical type of thyroid tumor,metastasis or not,and tumor locationexcept different gender and tumor location for IL-6 and different gender for IL-8 were significantly different(P< 0.05).The difference of the expression levels of IL-8 in <55years old and ≥ 55 years old,clinical type of thyroid tumor and metastasis were compared,P< 0.05,with statistical significance;The difference of the expression levels of TNF-α in different gender and metastasis except for <55 years old and ≥55 years old,clinical type of thyroid tumor and tumor location were significantly different(P < 0.05).4.The difference of the expression levels of VEGF,sFas,sFas L,IL-8 and TNF-α in thyroid cancer patients with different gender,< 55 years old and ≥55 years old,clinical type of thyroid tumor,metastasis or not,and tumor location after total thyroidectom was statistically significant(P < 0.05).The difference of the expression levels of IL-6 in < 55 years old and ≥55years old,clinical type of thyroid tumor,metastasis or not,and tumor location except for different gender were statistical y significant(P < 0.05).5.The findings show that the levels of VEGF,sFas and sFas L m RNA in blood of patients with thyroid cancer before and after operation was positively associated with the levels of serum IL-6,IL-8 and TNF-α.by Pearson correlation analysis.Conclusion1.The expression of VEGF,sFas and sFas L m RNA and the related IL-6,IL-8and TNF – α inflammatory factors were all lower than before the total thyroidectomy.It shows that after the thyroid tumor was removed,the molecular level of thyroid cancer patients improved the condition of the body and made the body recover to a certain extent.2.The expression of VEGF,sFas and sFas L were observed in patients with thyroid cancer of different sex,age,clinical type,metastasis and different tumor sites after total thyroidectomy.The expression of m RNA and the related IL-6,IL-8and TNF – α inflammatory factors were also lower than before thyroidectomy,and the reduction of the above indexes was different in different conditions,suggesting that there were also differences in the recovery of patients with different self conditions after total thyroidectomy.3.The levels of VEGF,sFas,sFas L m RNA in blood of patients with thyroid cancer before and after thyroidectomy were closely related to the levels of IL-6,IL-8and TNF-α in serum.It suggested that VEGF,sFas and sFas L m RNA levels were related to the inflammatory response of the body,and the total thyroidectomy could reduce the inflammatory response.