| Objective: TO study the gene expression patterns and research into measure of prevention and cure Of CNV.Methods: 1. 32 BN rats were randomly divided into 4 groups as 1 week, 2 weeks, 3 weeks and 4 weeks groups. The right eyes regarded as experimental eyes and left control eyes. Krypton laser photocoagulation was performed on the retina of experimental eyes to set up animal model. 1 week, 2 weeks, 3 weeks and 4 weeks after photocoagulation, FFA, HE, immunohistochemistry and quantitative analysis of CNV were executed. 2. 18 BN rats were randomly allocated 3 groups as 1 week, 2 weeks and 3 weeks groups. The right eyes regarded as experimental eyes and left control eyes. Krypton laser photocoagulation was performed on the retina of experimental eyes to set up animal model. 1 week, 2 weeks and 3 weeks after photocoagulation, the eyes were enucleated for harvesting choroidal tissue. Total RNA was abstracted from the choroidal tissue. The mRNA was purified and then was reversely transcribed to cDNA for hybridizaion probes. The differential expressed genes in choroidal tissue of experimental eyes and control eyes were assayed with gene chip. The data of gene chip were analyzed with Hierarchical clustering. 3. 24 BN rats after photocoagulation were randomly divided into 3 groups as GM6001 group, DMSO group and CONT group. 0.1% 0.2mL GM6001 suspension was injected in the behind eyeball for GM6001 group. 0.2mL DMSO was injected in the behind eyeball for DMSO group. The control eyes were not interfered.The retrobulbar injection was performed at day 1,5,9,13,17 after photocoagulation. 3 weeks after photocoagulation, FFA, HE, immunohistochemistry and quantitative analysis of CNV were performedResults: 1. 1 week after photocoagulation, FFA showed up disciform fluorescein leakage in the areas of photocoagulation; retinal edema, neutrophilic leukocytes, pigment macrophages, migratory and proliferative RPE cells,fibrocytes and CNV were found; immunohistochemistry for CD 105 displayed isolated positive staining in areas of photocoagulation; the area of CNV was(11.79±1.75 ) ×10~3μm~2. 2 weeks after photocoagulation, FFA showed up more fluorescein leakage in the areas of photocoagulation; retinal edema regressed; neutrophilic leukocytes disappeared; pigment macrophages decreased; fibrocytes increased; RPE cells proliferated and the areas of CNV increased. There were statistical differences for positive staining of immunohistochemistry for CD 105 from 1 week to 2 weeks (P<0.01) . The area of CNV was (9.72±2.50) ×10~3μm~2. There were statistical differences for the area of CNV from 1 week to 2 weeks (P<0.0\) . 3 weeks after photocoagulation, the fluorescein leakage little increased. Pigment macrophages were accidentally found; fibrocytes and RPE cells more proliferated; a great deal CNV formed. There were no statistical differences for positive staining of immunohistochemistry for CD 105 from 2 weeks to 3 weeks (P>0.05 ) . The area of CNV was (32.54±2.61) × 10~3μm~2. There were no statistical differences for the area of CNV from 2 weeks to 3 weeks. 4 weeks after photocoagulation, the fluorescein leakage was the same as 3 weeks. Pigment macrophages were not found; fibrocytes and migratory RPE cells more proliferated and a great deal CNV were enwrapped by fibrocytes and migratory and proliferative RPE cells. There were no statistical differences for positive staining of immunohistochemistry for CD 105 from 3 weeks to 4 weeks(P>0.05) . The area of CNV was (31.85±4.36) ×10~3μm~2. There were no statistical differences from 3 weeks to 4 weeks. 2.1 week after photocoagulation, the genes differential expressed have 427 genes. Of these, the expressions of 234 genes were upregulated and 193 genes downregulated. The expressions of MMP9, MMP12, COL1A1 and MMP2 were higher and MYH4, EEF2 and AQP5 lower. 2 weeks after photocoagulation, the genes differential expressed have 63 genes. Of these, the expressions of 45 genes were upregulated and 16 genes downregulated. The expressions of MMP2, ATP1B2 and ADD1 were higher and CTNNB1, GATMH and CRYBB2 lower. 3 weeks after photocoagulation, the genes differential expressed have 50 genes. Of these, the expressions of 8 genes were upregulated and 42 genes downregulated. The expressions of MMP9, MMP23 and MMP24 were higher and TNNI2, MYH4 and MYL2 lower. 3. 3 weeks after...
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