Studies On The Esterase Isozyme Of The Subfamily Tenebrioninae And Its Larval Systematics(Coleoptera: Tenebrionidae)

Tenebrioninae is a subfamily of tenebrionidae. Totally, there are 20 tribes in China. Adults of 14 tribes, 142 species from the northwest region were recorded by prof. Ren Guodong and prof. Yu Youzhi in 2003.The present paper is a study of the subfamily tenebrioninae from Mongolia-xingjiang region of China. It comprises four parts, namely, the part of summarization, esterase (EST) isozymes, subfamily tenebrioninae-larvae classfication part, discussion part.In the summarization part, the application of esterase isozymes electrophoresis technology in the systemat:c entomology, the history review of the classification of the subfamily i.enebrioninae-larvae and the classification system of tenebrionidae are introduced in details.In the esterase isozyme part, esterase isozymes in 23 species of the subfamily tenebrioninae-adult were studied by using the vertical slab polyacrylamide gel electrophoresis (PAGE) technology. The results are as follows:l.The number of esterase isozyme bands ranges from 9 to 23 in the 23 species. The relative mobility(Rf) of isozyme bands ranges from 0.13 to 0.98. There are many isozyme bands on anodic area and cathodic area.2. The zymograms variance among individuals and different sex is smaller than that of different species. Esterase isozymes differ sharply among different species , and each species has i ts own distinctive zymograms. The difference of zymograms in the different genera is more than in the same genera. The feasibility about using esterase isozymes in the systematic entomology is also discussed.3. The relationship of some species showing by cluster figure of EST isozyme zymogram Rm is certain similar with showing by morphological result.In the subfamily tenebrioninea-larvae classfication part, material and method, morphological character, biology anc economical importance are introduced. The morphology of the larvae is described. Totally ,83 species which belong to 7 tribes (Tenebrionini; Phaleriini; Crypticini; Pedinini; Opatrini; Platyscelini; Blaptini), 21 gener;i(TenebrJo Linnaeus, 1758; Parane Reitter, 1895; BlindusMulsant et Rey, 1953; Crypt/ayslatreille, 1817; Gonocephalum Solier, 1834; PenthicinusReitter, 1896; Myladina Reitter, 1889; EumyladaReitter, 1904; Mel'anest/iesDejean, 1834; Monatrum Reichardt, 1936; BioramixBates, 1879; AnatruvReichardt, 1936; Jintaium Ren, 1999; OpatrumFabricius, 1775; Pentfiicusl'aldermann, 1836; Oodescelis Motschusky, 1845; Platyscelis Latreille, 1825; Blaps Fabricius, 1775; Itagonia Reitter, 1887;Gnaptorina Reitter, 1887; Prosodes Eschscholtz, 1829) are thoroughly studied. Keys to the larvae of species and genera are given. Among them, 14 species are described in the first time. These species are as follows: Myladina sp. JintaiumsulcatumRen, 1999; Penthicus nojonicus Kaszab, 1968; Penthicns lenezyi Kaszab, 1968; Penthicus nanshanicus Reichardt, 1936; Penthicus sp. Melanesthes punctipennis Reitter, 1889, Melanesthes sp. Melanesthes rugipennis Reitter, 1889; Melanesthes JintaiensisRen, 1992; Melanesth es davadshamsi Kaszab, 1964; Melanesthes exillidentada Ren, 1993; Melanesthes ciliata Reitter, 1889; Blaps opaca Reitter, 1889.The larval classification of the subfamily tenebrioninae is discussed simply.All the specimens are preserved in Insoct Collection Agricultural College of Ningxia University.