Studies On Chemical Constituents Of Dracaena Cochinchinensis And Their Bioactivities

Dracaena cochinchinensis(Lour.) S. C. Chen. belongs to the genus Dracaena Vand. ex L, Liliaceae family. The red resin of D. cochinchinensis, called“longxuejie‖, is used as traditional Chinese dragon’s blood for hemostasis, analgesa, anti-bacterial, anti-inflammatory, analgesic, and anti-diabetic. The chemical constituents of D. cochinchinensis and their biotivities were studied to provide reference for the further research and application of this ethnomeicinal plants. This thesis consists of 4 chapters.Chapter 1. Advances in studies on chemical constituents and pharmacological activities of D. cochinchinensisIn this section, the chemical constituents of the resin and leaves from D. cochinchinensis are summarized, as well as the pharmacological activities.Chapter 2. Chemical constituents from Dracaena cochinchinensisIn order to find active compounds from this plant, the chemical constituents of the resin and leaves from D. cochinchinensis were investigated. Thirty-four compounds were isolated from the resin and leaves of D. cochinchinensis through a series of separation methods, including flavonoids, alkaloids, lignans, stilbenes, triterpenoid and steroidals. Based on various spectroscopic analysis(UV, IR, MS, 1D, 2D-NMR) and comparison of spectroscopic data with those in literatures, the structures of 28 compounds were determined. These compounds were identified as 3, 5-dihydroxy-2-methoxy-trans-stilbene(1), resveratrol(2), pterostilbene(3), pinostilbene(4),(3R)-3,7-dihydroxy-8-methoxy-3-(4’-methoxybenzyl)-4-chromanone(5), 4’-hydroxy-2, 4-dimethoxydihydrochalcone(6), 4, 4’-dihydroxy-2-methoxydihydro chalcone(7), 2, 4, 4’-trihydroxy-6-methoxydihydrochalcone(8), 4, 4’-dihydroxy-2, 6-dimethoxydihydrochalcone(9), 7, 4’-dihydroxy-8-methoxy-homoisoflavane(10), 7,4’-dihydroxyhomoisoflavane(11), 5,4’-dihydroxy-7-methoxy-6-methylflavane(12), 7-hydroxy-3-(4’-hydroxybenzyl)-4-chromanone(13), 3, 7-dihydroxy-8-methoxy-3-(4’-methoxybenzyl)-4-chromanone(14), 7-hydroxy-3-(4’-methoxybenzyl)-4-chromanone(15),(2S)-4’, 7-dihydroxy-3’-methoxy-8-methylflavan(16),(2S)-3’, 7-dihydroxy-4’-methoxy-8-methylflavan(17), 2’, 4, 4’-trihydroxylchalcone(18), N-cis-coumaroy tyramine(19), N-trans-feruloyl octopamine(20), N-trans-feruloyl tyramine(21), N-trans-coumaroy tyramine(22), 1, 2-diol-4- methoxy-benzene(23),(22E)-3β-acetoxystigmasta-5, 22-diene(24), lanost-9-en-3β-ol(25), β-sitosterol(26), β-daucosterol(27), pinoresinol(28). Among them, compound 14 is new, compound 1 is isolated as natural products for the first time. And compounds 19-20 were isolated from the genus Dracaena(Liliaceae) for the first time.Chapter 3. Bioactivities from Dracaena cochinchinensisIn this section, methanolic extracts and main compounds of the resin and leaves from D. cochinchinensis were tested for their anti-diabetic activities, anti-tumor activities and DPPH free radical scanvenging activities.The model of insulin resistance in HepG-2 cell was established to test the ameliorative effects of extracts and 14 compounds on glucose uptake. Compared with the model group, all compouds can significantly reduce the GLU contents in cell culture supernatants of insulin resistance celluar model. Compounds 1、4、5、6、8、9、12、20、21、22 showed different level of ameliorative effects on the insulin resistance in Hep G-2 cells at each concentration(10-6 to 10-5 or 10-5 to 10-4 mol/L) for 12 and 24 hours. Compouns 14、15、16、18 showed different different level of ameliorative effects at each concentration(10-5 to 10-4 mol/L) for 24 hours.Besides, tweenty compounds were tested for their anti-tumor activities in PC12,A549,MDA-MB-231 cells. Compounds 1、3、5、9、10、11 showed significant anti-tumor activities. Compounds 1、3、5、10、12 displayed relatively strong activity.Moreover, twenty-two compounds were tested for their abilities to scavenge 1, 1-diphenyl-2-picrylhydrazyl(DPPH) free radicals. Among them, compounds 1、14、16、17 displayed reasonable activities stronger than ascorbic acid and a-tocopherol. The compounds 20、21 showed relatively strong activities, equivalent to ascorbic acid and a-tocopherol.Chapter 4. Conclusion and ProspectsThe results studies on chemical constituents and bioactivity activities of D. cochinchinensis are summarized, as well as the future pharmaceutical development and utilization prospect of D. cochinchinensis.