| Tuberculosis (TB), caused by Mycobacterium tuberculosis and Mycobacterium bovis (M. bovis). M. bovis is main pathogens for causing animal bovine TB, such as cattle and deer et al. M. tuberculosis is the most common cause of human TB, but an unknown proportion of cases occur due to M. bovis. The evolution process of MTC is complex, so it is significant for studying on evolution process, stop and control TB's transmission.MTC can well-shielded from immune attack, and the culture is difficult, with the increase of clinical drug-resistant strains, especially the emergence of multidrug-resistant tuberculosis. The first, second-line anti-TB drugs gradually lose its effect. In China, traditional Chinese herbal medicine is an important source to treat common infections. Some of plants for the treatment of respiratory diseases have anti-TB activity. Use of traditional Chinese medicine to treat of Mycobacterium tuberculosis, is the future direction of development of TB, especially to multi-drug resistant tuberculosis control.In this study, we evaluate vitro anti-TB activity of 29 traditional Chinese herbal medicine, and 21 compounds which were isolated from medicinal plants. The result showed 4 herbal medicine and monomer compounds. Through microdilution alamar blue assay to detect drug sensitivity experiments for H37Rv. Based on the analysis, it is hoped to make an effort for finding gene targeting of the medicine.Gene chip technology with a high component, high-throughput and parallel can analyze thousands of genes simultaneously. It can be obtained within the scope of the whole genome of bacteria occurred in a series of changes, which stated at the genetic level, causing phenotypic differences reason, or find some interesting genes. Through the different time points before and after treatment to monitor gene expression levels, revealed the drug action pathway on the gene and its regulatory network, parsing mechanism of drug action, and access to the characteristics of the drug effect transcription spectrum. The aim of this study is identify the molecule mechanism of Osthole anti-MTC. We used minimum inhibitory concentration recommended by classical Committee for Clinical Laboratory Standards (CLSI), through microdilution flate drug sensitivity experiments, examining antibacterial effect. The strains are H37RV ATCC 27294 and clinical isolates. The MIC of Osthole is 0.125-0.625μg/mL. MTT cytotoxicity assay results showed that large do of Osthole≥32μg/mL is toxic on Vero cells. This dose is much higher than Osthole resistant clinical value of the MIC. the first time we use of Mycobacterium tuberculosis H37Rv (ATCC 27294) microarrays technology, assess from genome-wide level of H37RV ATCC 27294 gene expression effects by Osthole and find the molecular mechanism of anti-TB effect. The results show that standard sensitive strain H37RV ATCC 27294 induced by Osthole. There are 360 differentially expressed genes (expressed in multiples of≥1.5),161 up-regulated and 199 down, which involve Energy metabolism, DNA metabolism, Amino acid biosynthesis, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides and DNA interactions pathway by Osthole effects.The results of this study had identified the molecule mechanism of Osthole anti-MTC, it is a reference for finding gene targeting of traditional Chinese herbal medicine anti-MTC, and it can help rational administration to treat MTC by Chinese herbal medicine. This study is the first time to report the the molecule mechanism of Osthole anti-MTC, it is significant for traditional Chinese herbal medicine application.
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