Protective Effects Of Method Of Supplementing Qi And Activating Blood Circulation And Channels Combined With Mild-hypothermia In Cerebral Infarction Model Rats Of Qi-deficiency And Blood Stasis

Objective: To investigate the protective effects of method of supplementing qi and activating blood circulation and channels combined with mild- hypothermia in cerebral infarction model rats of qi-deficiency and blood stasis.Methods: SD rats were randomly divided into five groups: normal control group, model group, TCM group, mild-hypothermia group, combination therapy group. We observed the neurologic deficits and infarct sizes, pathologic changes, brain edema and brain tissue penetrability, nerve cell apoptosis and relating gene expression in cerebral infarction model rats of qi-deficiency and blood stasis.Result:1. The neurologic deficits and infarct sizes, brain edema and brain tissue penetrability, nerve cell apoptosis, bcl-2 and bax gene expression in model group were significantly higher than normal control group (p<0. 05).2. 2. Compared with model group, the neurologic deficits and infarct sizes, brain edema and brain tissue penetrability, nerve cell apoptosis, bax gene expression in all treatment group were significantly lower while bcl-2 gene expression was higher (p<0. 05).3. Compared between TCM group, mild-hypothermia group and combination therapy group, there was no remarkable difference in neurologic deficits and brain tissue penetrability. The infarct sizes, brain edema and the percentages of nerve cell apoptosis in combination therapy group were lower than that of TCM group or mild hypothermia group (p<0. 05). The percentage of nerve cell apoptosis in mild-hypothermia group was lower than that of TCM group (p<0. 05).4. The pathologic changes in all the treatment groups were obviously improved . The structure of nerve cell of normal control group was natural and no inflammatory cell was observed.Conclusion:1. We succeeded to make cerebral infarction model rats of qi-def iciency and blood stasis.2. Method of supplementing qi and activating blood circulation and channels and therapy of mild-hypothermia could provided protective effects in cerebral infarction of qi-deficiency and blood stasis respectively.3. Combination therapy was better than monotherapy (p<0. 05).4. Both two ways of therapy could decrease the apoptotic cells. This result may due to emhanced expression of bcl₂ and decreased expression of bax.