A Study Of The Clinic Effect And Mechanism Of JWZGY On Climacteric Syndrome

Research backgroundMenopause means the transition period which is from women's mature period tosenescence period, including menopause earlier stage, climacteric, menopause later stage. The phase that women enter menopause at the age of 41 to enter senescence period at the age of 60, which lasts about twenty years, occupied nearly 1/4 the life span of women. About 1/3 of women can reach the new balance through the self-regulation of the neural endocrine without having subjective symptoms in menopause. Two-thirds of the women appear a series of symptoms caused by sex hormone reducing, which is called climacteric syndrome.The Climacteric Syndrome is a gynecology common and frequently occurring disease, at present, regarded as by the decline of the ovary function in menopause, the estrogen changing makes the neural endocrine malfunction.The forepart mostly presents the blood vessel drawing back symptom, such as hectic fever, sweat etc. mainly mood shifts such as fret, melancholy, anxiety and etc. and the sex activity begins to fail; and then appears osteoporosis, memory decline, cognitive subside, cardiovascular and cerebral disease, old age dull-witted and Parkinson's, and the increasing of systematic disease as whole body endocrine, metabolism, immunity, breathing, urine, assimilation, cutis, facial features, and etc.The syndrome does big harm to the women's physical and mental health, working and living life. With social aging, the extension of the woman life span, the incidence of disease has the tendency to rise. Hence, people paid close attention to it day by day. Modern medical science mainly adopts the estrogen substituting treatment of the climacteric syndrome, or complement and jointly use medicine with the progestationalhormone, androgen. Although curative effect is assured, it cause bad reactions such as bleeding in vagina, breast pain and in a specified future it may cause uterus membrane cancer, breast cancer and etc. The side effect is unable to get rid of, so it is difficult to be accepted by the women, there is certain limitation in its application. Traditional Chinese medicine treatment of the climacteric syndrome has already been launched for many years in clinic, it can improve the clinical symptom and has no obvious poisonous side effect, and regulates such function in many aspects as internal system, nervous system, immune system, free radical metabolism. In recent years, much achievements have made by using the modern scientific and technical means, that is in studying and revealing the mechanism of the traditional Chinese medicine treatment of woman's the climacteric syndrome, and have disclosed the traditional Chinese medicine's regulating action on the patient's climacteric syndrome from such many aspects as the nerve, endocrine, immunity, and etc.Good effects has achieved in the clinical treatment of the climacteric syndrome by using JWZGY. This research is drafted through the clinical scientific research design method, appraise its clinical curative effect further, and discuss its function mechanism of treating this disease through animal's experiment, set solid foundation for further researching and developing the compound medicine made of two or more ingredients. Research objective1. Make clear the result of JWZGY in the treatment of the climacteric syndrome.2. Discuss the function mechanism of JWZGY in the treatment of the climacteric syndrome by animal experiments.Content of researchPart one: Literature studyConsult relevant literature materials in the past ten years, by the numbers review thestudy of the traditional Chinese medicine treatment of climacteric syndrome, that isthe cause of disease, clinical dialectical dividing type of syndrome and treatment;review the micro dialectical research combining traditional Chinese and westernmedicine in the treatment of climacteric syndrome; review the research of thepharmacology function mechanism, which are made of two or more ingredients oftraditional Chinese medicine to treat the climacteric syndrome; Put forward one's ownview about problems and countermeasures existing in the research, that is research ofthe traditional Chinese medicine treatment of the climacteric syndrome at present.Part two: the clinical studyChoose patient as the research object who is according with the diagnostic standard ofclimacteric syndrome, included standard, excluded standard. And divide them intotwo groups at random, the treat group use JWZGY and the contrast group are offeredNeil female alcohol block to select for use. Separately observe the influence thatJWZGY acted on women who have climacteric syndrome on the index such asKupperman's grade, sex hormone, and bone density.Part three: experimental researchesSelect the big mouse removal of its ovaries as the animal's model of syndromepathology in menopause, observe the following index:1> the influence of JWZGY on the level of serum E2^ FSI^ LH, BGP^ CT inovariectomized rats2 > the influence of JWZGY on bone density in ovariectomized rats3 n the influence of JWZGY on the bone histomorphometry in ovariectomized rats4^ the influence of JWZGY on the uterus weight and organ morphologic in ovariectomized rats5 -. the influence of JWZGY on the lower thalamus estrogen a receptor in ovariectomized rats Results Part one: Clinical study results1. JWZGY has effects on the improving various kinds of symptoms of the climacteric syndrome, Kupperman grades decreased significantly after using medicine. Among them, the improving effect on the patients suffering from insomnia, easy agitating, the bone joint muscle aches, is superior to that of the estrogen group.2. JWZGY can raise level of serum E2 of the woman who bearing the climacteric syndrome, but this function is obviously smaller than that of the estrogen; meanwhileJWZGY can reduce level of serum FSFK LH of the woman who have the climacteric syndrome significantly. Compared with this function and function of the estrogen, the difference has no significance.3. JWZGY can raise the density of women's oar and ulna end bones that have climacteric syndrome, and this function is slightly superior to that of the estrogen.4. The clinical curative effect of JWZGY is similar to that of estrogen. And there is no significant difference of affects comparing the availability totally release and estrogen.Part two: experiments results1. JWZGY has no obvious effect on the serum E2 level of old mice whose ovary are cut away, and there is an obvious difference between the function of it and estrogen; JWZGY can reduce the serum FSH% LH level in ovariectomized rats, the function will be less than that of estrogen when the JWZGY is in low or middle dosage, and the function will be equal to estrogen when the JWZGY is in high dosage.2. JWZGY can raise the serum BGP, CT level in ovariectomized rats; there is no significant difference of function comparing JWZGY's every dosage group and estrogen.3. JWZGY can raise the bone density in ovariectomized rats, which is the density of left and right thighbone of piphysis, waist of vertebra and this function is similar to function of the estrogen.4. JWZGY can obviously increase volume of trabecular bone and the average thickness of trabecular bone and bone cortex, dwindle interval, increase node and reduce the dissociate end number of trabecular bone, this function is* Similar to that of the estrogen.5. JWZGY can slightly increase uterus weight in ovariectomized rats, but this function is obviously lighter than that of the estrogen.6. JWZGY can delay the atrophy of membrane in the uterus in ovariectomized rats; this function is similar to estrogen.7. JWZGY can increase thalamus ERa in ovariectomized rats and its mRNA express.