| Objective:1. To observe the effects of electro-acupuncture dorsal L3～5 Jiaji points on skin blood flow in hind limbs of rats.2. To observe the effects of injecting capsaicin analogues (RTX), calcitonin gene related peptide 8-37, ATP sensitive K- channel antagonist glibenclamide, Ca2+ sensitive K+ channel antagonist scorpion toxins on skin blood flow during electro-acupuncturing Jiaji points of rats, and to investigate the relationship between electro-acupuncturing Jiaji points and the limb vasodilation by spinal cord stimulation.3. To discuss the mechanism of accumulatively electro-acupuncturing Jiaji points on vasodilation of rats hind limbs by counting the content changes of NO, ET-1,6-K-PGF1αand TXB2 in plasma, as well as the expression of eNOS and ET-1 in arterial wall.Methods:1. Conscious Wistar rats were fixed and stimulated on Jiaji points by general acupuncture and electro-acupuncture, respectively, and then the PU values of skin blood flow at different stages were compared.2. After electro-acupuncture stimulation on Jiaji points, the rats were injected capsaicin analogues (RTX), calcitonin gene related peptide 8-37, ATP sensitive K+ channel antagonist glibenclamide, Ca2+ sensitive K+ channel antagonist scorpion toxins, respectively, and then the PU values of skin blood flow before and after the injection were compared.3. The rats were randomly divided into two groups, blank group and electro-acupuncture group, and the electro-acupuncture group was daily stimulated on dorsal L3～5 Jiaji points for fifteen days. After that, the ELISA method was used to check the content of NO, ET-1,6-K-PGF1αand TXB2 of two groups.4. The rats were randomly divided into two groups, blank group, short-term electro-acupuncture group and long-term electro-acupuncture group, and the latter two groups were daily stimulated on dorsal L3～5 Jiaji points for seven and fifteen days, respectively. After that, the femoral arteries of three groups were taken and analyzed on the expression of eNOS and ET-1 by Western Blot technique. Results; 1. Electro-acupuncturing dorsal L3～5 Jiaji points can enhance the PU values of skin blood flow in hind limbs of rats.2. The enhancement of skin blood flow induced by electro-acupuncture can be inhabited by the injection of capsaicin analogues (RTX), calcitonin gene related peptide 8-37, glibenclamide, scorpion toxins.3. After accumulative stimulation, the contents of NO and 6-K-PGF1αin rats plasma were increased, while those of ET-1 and TXB2 were decreased; the expression of eNOS in hind limbs artery was enhanced, while that of ET-1 was weakened.Conclusions:Electro-acupuncturing dorsal L3～5 Jiaji points can enhance the vasodilation of rats hind limbs, which is quite similar to the result induced by spinal cord stimulation. Accumulative electro-acupuncture stimulation can change the contents of some vasoconstrictor and vasodilator, and can adjust endothelial cell function. Electro-acupuncture will be a novel treatment for PAOD disease in clinical practice.
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